Zhao Yanyan, Guo Liyan, Tian Jiajia, Wang Huafu
Department of Endocrinology, Weifang Yidu Central Hospital, Weifang, Shandong 262500, P.R. China.
Department of Clinical Pharmacy, Lishui People's Hospital, Lishui, Zhejiang 323000, P.R. China.
Exp Ther Med. 2021 Dec;22(6):1458. doi: 10.3892/etm.2021.10893. Epub 2021 Oct 19.
The aim of the present study was to investigate the regulatory effect and mechanism of microRNA (miR)-185 in diabetic angiopathy. The expression of miR-185 and nitric oxide synthase 2 (NOS2) in the blood from diabetic patients was examined by reverse transcription-quantitative PCR and enzyme-linked immunosorbent assay. After establishment of diabetic rats, the expression of miR-185 and NOS2 in vascular tissues and blood was also measured. Then, miR-185 was overexpressed in HMEC-1 cells and the expression of NOS2 was determined. Dual-luciferase reporter assay was used to identify the direct interaction between miR-185 and NOS2 mRNA. The expression of NOS2 was upregulated and the expression of miR-185 was downregulated in the blood from patients with diabetes. Vascular tissues and blood of diabetic rats showed similar trends compared with that of human. HMEC-1 cells with overexpression of miR-185 had decreased expression of NOS2. Dual-luciferase reporter assay demonstrated the direct binding between miR-185 and NOS2. The present study demonstrates that upregulation of NOS2 in diabetic patients is associated with the downregulation of miR-185, which participates in the progression of diabetes possibly through regulating NOS2 expression.
本研究旨在探讨微小RNA（miR）-185在糖尿病性血管病变中的调控作用及机制。采用逆转录定量聚合酶链反应和酶联免疫吸附测定法检测糖尿病患者血液中miR-185和一氧化氮合酶2（NOS2）的表达。建立糖尿病大鼠模型后，检测血管组织和血液中miR-185和NOS2的表达。然后，在人微血管内皮细胞-1（HMEC-1）中过表达miR-185并测定NOS2的表达。采用双荧光素酶报告基因测定法鉴定miR-185与NOS2 mRNA之间的直接相互作用。糖尿病患者血液中NOS2表达上调，miR-185表达下调。与人类相比，糖尿病大鼠的血管组织和血液呈现相似趋势。miR-185过表达的HMEC-1细胞中NOS2表达降低。双荧光素酶报告基因测定法证实miR-185与NOS2之间存在直接结合。本研究表明，糖尿病患者中NOS2的上调与miR-185的下调有关，miR-185可能通过调节NOS2表达参与糖尿病的进展。
