Costa Diana, Valente Artur J M, Queiroz João
CICS-UBI - Health Sciences Research Centre, University of Beira Interior, Av. Infante D. Henrique, 6200-506 Covilhã, Portugal.
CQC, Department of Chemistry, University of Coimbra, 3004-535 Coimbra, Portugal.
J Biotechnol. 2015 May 20;202:98-104. doi: 10.1016/j.jbiotec.2014.10.033. Epub 2014 Nov 20.
This study provides a detailed description on the synthesis and characterization of novel polyamine plasmid DNA nanogels. Ethylene glycol diglycidyl ether was used as cross-linker, in conjugation with polyamines to promote pDNA condensation. The biocompatible nanovectors exhibit a unique swelling behavior in water and salt solutions. These systems are light photodegradable allowing their use in a broad range of biotechnological applications. Different plasmids, pVAX1-LacZ and pcDNA3-FLAG-p53, and anticancer drugs were, thus, efficiently loaded in the nanogels and their controlled release was demonstrated. Furthermore, the dual delivery of pcDNA3-FLAG-p53 gene and anticancer drugs illustrates the possibility of the combination of chemical and gene therapies. This new versatile and easy method of nanohydrogels preparation provides a potential synthetic approach for the design of tunable systems which can display multiple functions, sensitivity to different stimuli and exhibit programmed responses as well.
本研究详细描述了新型多胺质粒DNA纳米凝胶的合成与表征。乙二醇二缩水甘油醚用作交联剂，与多胺结合以促进pDNA凝聚。这种生物相容性纳米载体在水和盐溶液中表现出独特的溶胀行为。这些体系具有光可降解性，可用于广泛的生物技术应用。因此，不同的质粒pVAX1-LacZ和pcDNA3-FLAG-p53以及抗癌药物被有效地负载到纳米凝胶中，并证明了它们的控释性能。此外，pcDNA3-FLAG-p53基因和抗癌药物的双重递送说明了化学疗法和基因疗法联合应用的可能性。这种制备纳米水凝胶的新型通用且简便的方法为设计可调谐系统提供了一种潜在的合成方法，该系统可显示多种功能、对不同刺激敏感并表现出程序化响应。
