von Charpuis Charlotte, Meckel Tobias, Moroni Anna, Thiel Gerhard
Plant Membrane Biophysics, TU Darmstadt, Schnittspahnstrasse 3, 64287 Darmstadt, Germany.
Department of Biology and CNR IBF-Mi, and Istituto Nazionale di Fisica della Materia, Università degli Studi di Milano, Via Celoria 26, 20133 Milano, Italy.
Cell Calcium. 2015 Jul;58(1):114-21. doi: 10.1016/j.ceca.2014.09.009. Epub 2014 Oct 5.
The two small and similar viral K(+) channels Kcv and Kesv are sorted in mammalian cells and yeast to different destinations. Analysis of the sorting pathways shows that Kcv is trafficking via the secretory pathway to the plasma membrane, while Kesv is inserted via the TIM/TOM complex to the inner membrane of mitochondria. Studies with Kesv mutants show that an N-terminal mitochondrial targeting sequence in this channel is neither necessary nor sufficient for sorting of Kesv the mitochondria. Instead the sorting of Kesv can be redirected from the mitochondria to the plasma membrane by an insertion of ≥2 amino acids in a position sensitive manner into the C-terminal transmembrane domain (TMD2) of this channel. The available data advocate the presence of a C-terminal sorting signal in TMD2 of Kesv channel, which is presumably not determined by the length of this domain.
两种小型且相似的病毒钾离子通道Kcv和Kesv在哺乳动物细胞和酵母中被分选到不同的目的地。对分选途径的分析表明，Kcv通过分泌途径运输到质膜，而Kesv通过TIM/TOM复合体插入到线粒体内膜。对Kesv突变体的研究表明，该通道中的N端线粒体靶向序列对于Kesv分选到线粒体既不是必需的也不是充分的。相反，通过以位置敏感的方式在该通道的C端跨膜结构域（TMD2）中插入≥2个氨基酸，Kesv的分选可以从线粒体重新定向到质膜。现有数据支持在Kesv通道的TMD2中存在C端分选信号，这可能不是由该结构域的长度决定的。
