Raghu Patil J, Desai Srividya Narayanamurthy, Roy Panchali, Durgaiah Murali, Saravanan R Sanjeev, Vipra Aradhana
Microbiology, In vivo, and Molecular Biology Team, Gangagen Biotechnologies Pvt. Ltd., No. 12, 5th Cross, Raghavendra layout, Opp. MEI Ltd., Tumkur Road, Yeshwantpur, Bangalore-560022, India.
Dis Aquat Organ. 2014 Dec 2;112(2):113-9. doi: 10.3354/dao02806.
Vibriosis caused by luminous Vibrio harveyi commonly contributes to poor survival in shrimp hatcheries and aquaculture ponds. Lytic bacteriophages pathogenic for V. harveyi are currently being investigated as an alternative to antibiotics to prevent vibriosis. Here, 8 bacteriophages were isolated from oysters and clams using V. harveyi strains as baiting hosts. Among these bacteriophages, 1 strain (VHP6b) identified as broadly pathogenic for 27 V. harveyi strains examined was further characterized by electron microscopy and genome sequence analysis. Phage VHP6b possessed a tail and morphology consistent with it being a member of the family Siphoviridae, and its genome and proteome were most closely related to the Vibrio phages SSP02 and MAR10. An integrase gene essential for lysogeny was not evident. The ability of bacteriophage VHP6b to protect shrimp postlarvae against vibriosis caused by V. harveyi strain VH6 was demonstrated in a model system designed to simulate typical hatchery conditions. Bacteriophage treatment improved survival of postlarvae by 40 to 60% under these conditions, so therapies based on this or other bacteriophages may be useful in shrimp hatcheries.
由发光哈维氏弧菌引起的弧菌病通常会导致虾苗孵化场和养殖池塘中虾的存活率低下。目前正在研究对哈维氏弧菌具有致病性的裂解性噬菌体，以替代抗生素来预防弧菌病。在此，以哈维氏弧菌菌株作为诱饵宿主，从牡蛎和蛤中分离出8种噬菌体。在这些噬菌体中，有1株（VHP6b）被鉴定为对所检测的27株哈维氏弧菌具有广泛致病性，通过电子显微镜和基因组序列分析对其进行了进一步表征。噬菌体VHP6b具有一个尾部，其形态与它属于长尾噬菌体科的成员一致，并且其基因组和蛋白质组与弧菌噬菌体SSP02和MAR10的关系最为密切。未发现与溶原性必需的整合酶基因。在一个旨在模拟典型孵化场条件的模型系统中，证明了噬菌体VHP6b保护虾幼体免受哈维氏弧菌菌株VH6引起的弧菌病的能力。在这些条件下，噬菌体处理使幼体的存活率提高了40%至60%，因此基于这种或其他噬菌体的疗法可能对虾苗孵化场有用。
