Pasharawipas Tirasak, Thaikua Surasak, Sriurairatana Siriporn, Ruangpan Lila, Direkbusarakum Sataporn, Manopvisetcharean Jaroon, Flegel Timothy W
Department of Microbiology, Faculty of Science, Rangsit University, Pathumthani 12000, Thailand.
Virus Res. 2005 Dec;114(1-2):63-9. doi: 10.1016/j.virusres.2005.05.012. Epub 2005 Jul 14.
A bacteriophage was isolated together with Vibrio harveyi (VH) 1114 a from a black tiger shrimp-rearing pond in Thailand. By negative staining transmission electron microscopy (TEM), the phage had an icosahedral head (diameter 60-62 nm), a rigid, non-contractile tail (9-10 nm x 100-120 nm) without a collar or terminal fibers and a genome of double stranded DNA of approximately 80 kb as determined by analysis of restriction enzyme digestion fragments. Since these features would place it in the family Siphoviridae, it was tentatively named V. harveyi siphoviridae-like phage or VHS1. VHS1 could also infect two VH reference strains LMD 22.30 and LMD 80.33 (=ATCC 14126) but yielded smaller plaques than with VH1114. The phage tolerated temperatures as high as 60 degrees C for up to 2h and overnight exposure to a broad range of pH. VHS1 lysogens of VH1114 were unstable, contained unaltered VHS1 DNA, were immune to VHS1 lysis and spontaneously released VHS1 in liquid cultures. Approximately 20 kb of the genome has been sequenced and deposited at GenBank but it mostly showed no significant homology with existing sequences in the database.
从泰国一个黑虎虾养殖池塘中，分离出了一种噬菌体，同时还分离出了哈维氏弧菌（VH）1114a。通过负染色透射电子显微镜（TEM）观察，该噬菌体有一个二十面体头部（直径60 - 62纳米），一条刚性的、不可收缩的尾部（9 - 10纳米×100 - 120纳米），没有颈环或末端纤维，经限制性内切酶消化片段分析确定其基因组为双链DNA，约80千碱基对。由于这些特征表明它属于长尾噬菌体科，因此暂时将其命名为哈维氏弧菌类长尾噬菌体或VHS1。VHS1也能感染两种VH参考菌株LMD 22.30和LMD 80.33（=ATCC 14126），但形成的噬菌斑比感染VH1114时小。该噬菌体在高达60摄氏度的温度下耐受2小时，并且能在广泛的pH范围内过夜存活。VH1114的VHS1溶原菌不稳定，含有未改变的VHS1 DNA，对VHS1裂解具有免疫性，并且在液体培养物中能自发释放VHS1。已对约20千碱基对的基因组进行了测序并提交到GenBank，但它与数据库中现有的序列大多没有显著同源性。
