Yang Xia, Guo Jian Lin, Ye Jin Yun, Zhang Yi Xiang, Wang Wei
Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, College of Life Science, Huzhou University, Huzhou, Zhejiang, 313001, China.
Zhejiang Institute of Freshwater Fisheries, Huzhou, Zhejiang, 313001, China.
Fish Shellfish Immunol. 2015 Jan;42(1):132-7. doi: 10.1016/j.fsi.2014.10.037. Epub 2014 Nov 7.
The present study investigated the effect of Ficus carica polysaccharide (FCP), isolated from the fruit of F. carica L., at 0%, 0.1%, 0.5% and 1.0% doses supplementation with feed on genes Interleukin 1-β (IL-1β), Tumor Necrosis Factor α (TNF-α) and heat shock protein 70 (HSP70) gene expression in blood, humoral innate immune parameters and resistant to Flavobacterium columnare of grass carp at weeks 1, 2 and 3. The results revealed that administration of FCP significantly (P<0.05) up regulated IL-1β and TNF-α gene expression. HSP70 gene expression was significantly (P<0.05) lower in FCP-fed fish at the end of trial. The serum total protein, albumin and globulin did not significantly increased in any diet on the first week whereas it was significantly enhanced in 0.5% and 1.0% supplementation diets on weeks 2 and 3 when compared to control. The serum complement C3 was significantly (P<0.05) increased on weeks 1 and 2 when compared to control, however, no significant difference was found in this activity after 3 weeks of treatment. All diets significantly enhanced the serum lysozyme activity, bactericidal activity from weeks 1-2 as compared to control. Grass carp fed with FCP showed remarkably higher resistance against F. columnare (60% survival) compared to the control group (30% survival). These results confirm that FCP can up regulate immune related genes expression, stimulates immune response that per se enhances disease resistance in grass carp.
本研究调查了从无花果果实中分离出的无花果多糖（FCP），以0%、0.1%、0.5%和1.0%的剂量添加到饲料中，对草鱼在第1、2和3周时血液中白细胞介素1-β（IL-1β）、肿瘤坏死因子α（TNF-α）和热休克蛋白70（HSP70）基因表达、体液固有免疫参数以及柱状黄杆菌抗性的影响。结果显示，给予FCP显著（P<0.05）上调了IL-1β和TNF-α基因表达。试验结束时，喂食FCP的鱼中HSP70基因表达显著（P<0.05）降低。第一周时，任何饲料组的血清总蛋白、白蛋白和球蛋白均未显著增加，而与对照组相比，在第2和3周时，0.5%和1.0%添加量的饲料组中这些指标显著升高。与对照组相比，血清补体C3在第1和2周时显著（P<0.05）增加，但在处理3周后该活性未发现显著差异。与对照组相比，所有饲料在第1至2周均显著提高了血清溶菌酶活性和杀菌活性。喂食FCP的草鱼对柱状黄杆菌的抗性明显高于对照组（存活率60%）（对照组存活率30%）。这些结果证实，FCP可上调免疫相关基因表达，刺激免疫反应，从而增强草鱼的抗病能力。
