College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin 130118, China.
Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin 130102, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Fish Shellfish Immunol. 2018 Jan;72:604-610. doi: 10.1016/j.fsi.2017.11.027. Epub 2017 Nov 13.
The objective was to add 0, 400, 800 or 1200 mg/kg of Hericium caput-medusae polysaccharide (HCMP) to the basal diet of grass carp (Ctenopharyngodon idella) and determine effects on humoral innate immunity, expression of immune-related genes and disease resistance. Adding HCMP enhanced (P < 0.05) bactericidal activity at 1, 2 and 3 weeks and also lysozyme activity, complement C3, and SOD activity at 2 and 3 weeks. Supplementing 800 or 1200 mg/kg of HCMP for 2 or 3 weeks increased (P < 0.05) serum concentrations of total protein, albumin and globulin. Two immune-related genes (IL-1β and TNF-α) were up-regulated (P < 0.05) in HCMP supplemented groups given 800 or 1200 mg/kg HCMP after 2 and 3 weeks of feeding. Expression of anti-inflammatory cytokine IL-10 was down-regulated (P < 0.05) after receiving 800 or 1200 mg/kg HCMP for 2 or 3 weeks. Fish fed 800 mg/kg HCMP had maximal disease resistance against Aeromonas hydrophila (65.4%). In conclusion, HCMP enhanced immune response and expression of immune-related genes and increased disease resistance against Aeromonas hydrophila in grass carp, with greatest effects in fish given 800 mg/kg HCMP for 3 weeks.
本研究的目的是在草鱼（Ctenopharyngodon idella）基础日粮中添加 0、400、800 或 1200mg/kg 猴头菇多糖（HCMP），并测定其对体液固有免疫、免疫相关基因表达和抗病力的影响。添加 HCMP 可增强（P<0.05）1、2 和 3 周时的杀菌活性，以及 2 和 3 周时的溶菌酶活性、补体 C3 和 SOD 活性。在 2 或 3 周内补充 800 或 1200mg/kg 的 HCMP 可增加（P<0.05）血清总蛋白、白蛋白和球蛋白浓度。2 和 3 周时，添加 800 或 1200mg/kg HCMP 的草鱼中两种免疫相关基因（IL-1β和 TNF-α）的表达上调（P<0.05）。在接受 800 或 1200mg/kg HCMP 处理 2 或 3 周后，抗炎细胞因子 IL-10 的表达下调（P<0.05）。连续 3 周投喂 800mg/kg HCMP 的草鱼对嗜水气单胞菌的抗病力最高（65.4%）。综上所述，HCMP 增强了草鱼的免疫反应和免疫相关基因的表达，并提高了其对嗜水气单胞菌的抗病力，其中投喂 800mg/kg HCMP 3 周的效果最佳。
