用于检测针对com1基因的伯氏考克斯体的环介导等温扩增检测法。
Loop-mediated isothermal amplification assay for detection of Coxiella burnetii targeting the com1 gene.
作者信息
Das Durga Prasad, Malik Satya Veer Singh, Sahu Radhakrishna, Yadav Jay Prakash, Rawool Deepak B, Barbuddhe Sukhadeo B
机构信息
Division of Veterinary Public Health, ICAR- Indian Veterinary Research Institute, Izatnagar 243 122, India.
ICAR-National Research Centre on Meat, Hyderabad 500092, India.
出版信息
J Microbiol Methods. 2018 Dec;155:55-58. doi: 10.1016/j.mimet.2018.11.011. Epub 2018 Nov 14.
We developed the com1 gene based loop-mediated isothermal amplification (LAMP) assay for the detection of Coxiella burnetii and validated it by screening DNA isolated from serum samples collected from animals and humans. The detection of Coxiella by LAMP assay was comparable with the com1 based-PCR.
我们开发了基于com1基因的环介导等温扩增（LAMP）检测法用于检测伯氏考克斯体，并通过筛选从动物和人类采集的血清样本中分离的DNA对其进行了验证。通过LAMP检测法检测伯氏考克斯体与基于com1的聚合酶链反应（PCR）相当。
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