Rueda Cesar M, Wells Casey B, Gisslen Tate, Jobe Alan H, Kallapur Suhas G, Chougnet Claire A
Division of Immunobiology, Cincinnati Children's Hospital Research Foundation, Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
Division of Neonatology/Pulmonary Biology, The Perinatal Institute, Cincinnati Children's Hospital Medical Center, University of Cincinnati, OH, USA.
Hum Immunol. 2015 Jan;76(1):65-73. doi: 10.1016/j.humimm.2014.10.016. Epub 2014 Nov 8.
Regulatory T-cells (Treg) have a protective role for the control of immune activation and tissue damage. The effects of chorioamnionitis (chorio) on Treg in moderate/late preterm newborns are not known. We hypothesized that infants exposed to chorio would have decreased Treg frequency and/or function. We isolated mononuclear cells from adult peripheral blood and cord blood from term and moderate/late preterm infants who were classified for severity of chorio exposure. Mononuclear cells were analyzed by flow cytometry for Treg frequency and phenotype. Treg suppression of activation of conventional T-cells (Tcon) was also quantified. Treg frequencies were similar in all groups of neonates, but lower than that found in adults. Newborn Treg had a naïve phenotype, with decreased levels of CD45RO, HLA-DR, CD39 and TIGIT compared to adult Treg and chorio did not affect the phenotype. Treg from preterm newborns exposed to severe chorio had higher expression of Ki67 compared to the other groups. Treg from preterm newborns were less suppressive than Treg from adults or term, and the level of suppression was reduced with severe chorio. Relative to term, Treg frequency and phenotype were not affected by prematurity and chorio but their functionality was decreased. Lower Treg activity may contribute to inflammation in newborns that is often associated with chorioamnionitis.
调节性T细胞（Treg）在控制免疫激活和组织损伤方面具有保护作用。绒毛膜羊膜炎（chorio）对中度/晚期早产儿Treg的影响尚不清楚。我们假设暴露于绒毛膜羊膜炎的婴儿Treg频率和/或功能会降低。我们从足月和中度/晚期早产儿的成人外周血和脐带血中分离单核细胞，这些婴儿根据绒毛膜羊膜炎暴露的严重程度进行分类。通过流式细胞术分析单核细胞的Treg频率和表型。还对Treg对常规T细胞（Tcon）激活的抑制作用进行了量化。所有新生儿组的Treg频率相似，但低于成人。新生儿Treg具有幼稚表型，与成人Treg相比，CD45RO、HLA-DR、CD39和TIGIT水平降低，绒毛膜羊膜炎不影响表型。与其他组相比，暴露于严重绒毛膜羊膜炎早产儿的Treg Ki67表达更高。早产儿的Treg比成人或足月儿的Treg抑制作用更小，严重绒毛膜羊膜炎会降低抑制水平。相对于足月儿，Treg频率和表型不受早产和绒毛膜羊膜炎影响，但其功能降低。较低的Treg活性可能导致新生儿炎症，这通常与绒毛膜羊膜炎有关。
