1型糖尿病中冷冻保存的CD4(+)CD25(+)CD127(lo/-)细胞的高效扩增
Efficient expansion of cryopreserved CD4(+)CD25(+)CD127(lo/-) cells in Type 1 diabetes.
作者信息
Rydén Anna, Faresjö Maria
机构信息
Division of Paediatrics & Diabetes Research Centre, Department of Molecular & Clinical Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden.
Division of Paediatrics & Diabetes Research Centre, Department of Molecular & Clinical Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden ; Department of Natural Science and Biomedicine, School of Health Sciences, Jönköping University, Jönköping, Sweden.
出版信息
Results Immunol. 2011 Aug 22;1(1):36-44. doi: 10.1016/j.rinim.2011.08.001. eCollection 2011.
Increased attention has been drawn to the important role played by regulatory T-cells (Treg) in immune homoeostasis. However, the small numbers of Tregs make them elusive to study. We investigated the cryostability of Tregs and whether they can be expanded from cryopreserved peripheral blood mononuclear cells (PBMCs). Further, to elucidate if there is a difference in ex-vivo frequency or in vitro expansion of Tregs among T1D children (n=9), high-risk (n=7) and healthy (n=10) individuals, Tregs defined as CD4(+)CD25(+)CD127(lo/-) were isolated from cryopreserved PBMCs. Cryopreserved PBMCs maintained a stable expression of Treg-markers. Tregs were efficiently expanded in vitro from all donors and Tregs from T1D children acquired higher FOXP3 expression compared to healthy subjects. T1D children had a significantly lower percentage of Tregs among CD4(+) T-cells and also lower Treg to CD4(+)CD25(-) cell ratios compared to healthy individuals.
调节性T细胞（Treg）在免疫稳态中发挥的重要作用已引起越来越多的关注。然而，Treg数量较少，难以进行研究。我们研究了Treg的冷冻稳定性以及它们是否可以从冷冻保存的外周血单个核细胞（PBMC）中扩增出来。此外，为了阐明1型糖尿病儿童（n = 9）、高危个体（n = 7）和健康个体（n = 10）之间Treg的体外频率或体外扩增是否存在差异，从冷冻保存的PBMC中分离出定义为CD4(+)CD25(+)CD127(lo/-)的Treg。冷冻保存的PBMC维持了Treg标志物的稳定表达。所有供体的Treg在体外均能有效扩增，与健康受试者相比，1型糖尿病儿童的Treg获得了更高的FOXP3表达。与健康个体相比，1型糖尿病儿童CD4(+) T细胞中Treg的百分比显著降低，Treg与CD4(+)CD25(-)细胞的比例也更低。
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