Yamamoto Yukihiro, Mizuta Eri, Ito Mizue, Harata Masataka, Hiramoto Shigeru, Hara Setsuko
Faculty of Science and Technology, Seikei University.
J Oleo Sci. 2014;63(12):1275-81. doi: 10.5650/jos.ess14125.
To utilize n-3 polyunsaturated fatty acid (PUFA) for a wide range of applications, we prepared phospholipids (PLs) containing PUFAs as constituent fatty acids (PUFA-PLs) via commercially available lipase OF-mediated transacylation with PL from soy (Soy-PL) and ethyl ester of PUFA (PUFA-Et). In a preliminary study to evaluate PUFA-incorporation (wt%) on phosphatidylcholine (PC), we observed that dehydration of Soy-PL is critical. PUFA-incorporation in PLs increased with acyl ratio and time. Finally, maximum PUFA-incorporation (47.1 ± 2.1 wt%) was obtained using the following reaction conditions: 2.0 mmol of Soy-PL, a PUFA-Et/Soy-PL acyl ratio of 7, 13 mL of hexane, 2.2 × 10(5) U of lipase OF, 500 rpm of agitation, a temperature of 37°C, and 72 h of reaction time. The analysis of fatty acid composition at the sn-2 position of obtained PL revealed that PUFAs incorporated into Soy-PL localized to the sn-2 position of the PL molecule in spite of using lipase OF whose positional specificity is random for triacylglycerol.
为了将n-3多不饱和脂肪酸（PUFA）用于广泛的应用，我们通过市售脂肪酶OF介导的大豆磷脂（Soy-PL）与PUFA乙酯（PUFA-Et）的转酰基作用，制备了含有PUFA作为组成脂肪酸的磷脂（PLs）（PUFA-PLs）。在一项评估PUFA掺入量（重量%）对磷脂酰胆碱（PC）影响的初步研究中，我们观察到Soy-PL的脱水至关重要。PLs中PUFA的掺入量随酰基比率和时间增加。最后，使用以下反应条件获得了最大PUFA掺入量（47.1±2.1重量%）：2.0 mmol的Soy-PL，PUFA-Et/Soy-PL酰基比率为7，13 mL己烷，2.2×10⁵ U的脂肪酶OF，500 rpm搅拌速度，37°C温度，以及72 h反应时间。对所得PL的sn-2位脂肪酸组成分析表明，尽管使用的脂肪酶OF对三酰甘油的位置特异性是随机的，但掺入Soy-PL的PUFAs定位于PL分子的sn-2位。
