Pushkarev V M, Tron'ko N D, Mikosha A S
Biokhimiia. 1989 Feb;54(2):323-7.
The role of cAMP in K+-dependent regulation of aldosterone output was studied. Guinea pig adrenal slices were incubated with various concentrations of K+ for 20 min. Aldosterone and cAMP output, protein synthesis and phosphorylation were increased with a rise in K+ concentration. The increase in the phosphorylation of the protein with apparent molecular weight of about 38 kD resulted from the increase in K+ concentration in the incubation medium. Proteins from adrenal slices preincubated with 3.0 and 11.0 mM K+ were separated by SDS polyacrylamide gel electrophoresis. No qualitative differences were revealed in autoradiograms of [14C]-prelabeled proteins; however, quantitative differences were present. These results suggest that the cAMP messenger system may be involved in the K+-dependent control of aldosterone biosynthesis.
研究了环磷酸腺苷（cAMP）在钾离子依赖性醛固酮分泌调节中的作用。将豚鼠肾上腺切片与不同浓度的钾离子孵育20分钟。随着钾离子浓度的升高，醛固酮和cAMP的分泌、蛋白质合成及磷酸化均增加。孵育介质中钾离子浓度的升高导致表观分子量约为38kD的蛋白质磷酸化增加。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离预先用3.0和11.0mM钾离子孵育的肾上腺切片中的蛋白质。在[14C]预标记蛋白质的放射自显影片中未发现定性差异；然而，存在定量差异。这些结果表明，cAMP信使系统可能参与了钾离子依赖性醛固酮生物合成的控制。
