Pushkarev V M, Tron'ko N D, Mikosha A S
Biokhimiia. 1987 Jul;52(7):1174-9.
The effect of K+ concentration on protein biosynthesis and 32P-incorporation into the acid-insoluble fraction of dog and guinea pig adrenal cortex slices was studied. An increase in K+ concentration in the incubation medium from 3 to 8-11 mM induced after 15-20 min of incubation a significant stimulation of 14C-leucine incorporation into the acid-insoluble fraction of post-mitochondrial supernatant. More extensive labelling of this fraction with 32P was observed. Addition of valinomycin caused a shift in the maximum of 14C-leucine incorporation towards lower K+ concentrations. The Na+,K+-ATPase inhibitors--ouabain and strophantin K--reduced the K+-stimulated protein synthesis. These data suggest that K+ transport into the cell is necessary for the stimulating effect to be manifested. Chelation of Ca2+ strongly decreased the incorporation of 14C-leucine into proteins in the presence of 5 mM K+. However, protein labelling increased with a gradual rise in K+ concentration up to 25 mM.
研究了钾离子浓度对狗和豚鼠肾上腺皮质切片蛋白质生物合成以及32P掺入酸不溶性部分的影响。孵育培养基中钾离子浓度从3 mM增加到8 - 11 mM，孵育15 - 20分钟后，显著刺激了14C - 亮氨酸掺入线粒体后上清液的酸不溶性部分。观察到该部分有更广泛的32P标记。加入缬氨霉素使14C - 亮氨酸掺入的最大值向较低钾离子浓度方向移动。钠离子，钾离子 - ATP酶抑制剂——哇巴因和毒毛旋花子苷K——降低了钾离子刺激的蛋白质合成。这些数据表明钾离子转运进入细胞是表现出刺激作用所必需的。在存在5 mM钾离子的情况下，螯合钙离子强烈降低了14C - 亮氨酸掺入蛋白质的量。然而，随着钾离子浓度逐渐升高至25 mM，蛋白质标记增加。
