Department of Cell Biology, Johns Hopkins University School of Medicine, 725 N. Wolfe Street, Baltimore, MD 21205, USA.
Department of Biology, College of William and Mary, Williamsburg, VA 23185, USA.
Dev Cell. 2014 Nov 24;31(4):474-86. doi: 10.1016/j.devcel.2014.10.004. Epub 2014 Nov 13.
Local signals maintain adult stem cells in many tissues. Whether the sexual identity of adult stem cells must also be maintained was not known. In the adult Drosophila testis niche, local Jak-STAT signaling promotes somatic cyst stem cell (CySC) renewal through several effectors, including the putative transcription factor Chronologically inappropriate morphogenesis (Chinmo). Here, we find that Chinmo also prevents feminization of CySCs. Chinmo promotes expression of the canonical male sex determination factor DoublesexM (Dsx(M)) within CySCs and their progeny, and ectopic expression of DsxM in the CySC lineage partially rescues the chinmo sex transformation phenotype, placing Chinmo upstream of Dsx(M). The Dsx homolog DMRT1 prevents the male-to-female conversion of differentiated somatic cells in the adult mammalian testis, but its regulation is not well understood. Our work indicates that sex maintenance occurs in adult somatic stem cells and that this highly conserved process is governed by effectors of niche signals. PAPERCLIP:
局部信号在许多组织中维持成年干细胞。然而，人们并不知道成年干细胞的性别身份是否也需要维持。在成年果蝇睾丸小生境中，局部 Jak-STAT 信号通过几个效应物促进体腔干细胞 (CySC) 的更新，包括假定的转录因子 Chronologically inappropriate morphogenesis (Chinmo)。在这里，我们发现 Chinmo 还可以防止 CySCs 的雌性化。Chinmo 在 CySCs 及其后代中促进经典雄性性别决定因子 DoublesexM (Dsx(M)) 的表达，并且在 CySC 谱系中外源表达 DsxM 部分挽救了 chinmo 性别转化表型，将 Chinmo 置于 Dsx(M)的上游。同源物 DMRT1 阻止成年哺乳动物睾丸中已分化体细胞的雄性到雌性的转化，但它的调控机制尚不清楚。我们的工作表明，性别维持发生在成年体干细胞中，并且这个高度保守的过程受小生境信号效应物的调控。
