Irminger J C, Schoenle E J, Briner J, Humbel R E
Department of Biochemistry, University of Zürich, Switzerland.
Eur J Pediatr. 1989 Jun;148(7):620-3. doi: 10.1007/BF00441514.
In this study messenger ribonucleic acid (mRNA) and DNA of five Wilms tumours were investigated. As expected, the level of insulin-like growth factor (IGF) II-mRNA was elevated up to 50 times in tumour tissue as compared to normal adjacent kidney tissue. In addition, genomic DNA was isolated and digested with appropriate restriction enzymes. Southern blots were prepared and hybridized to IGF II-cDNA probes. Additional bands were present in one of the five Wilms tumours compared to normal tissue. The results indicate a rearrangement of the IGF II-gene on one of the two chromosomes. It is speculated, that this change is responsible for the elevated IGF II expression which may be a factor contributing to tumour growth.
在本研究中，对5例肾母细胞瘤的信使核糖核酸（mRNA）和DNA进行了研究。正如预期的那样，与相邻正常肾组织相比，肿瘤组织中胰岛素样生长因子（IGF）II-mRNA水平升高了50倍。此外，分离基因组DNA并用适当的限制性内切酶进行消化。制备Southern印迹并与IGF II-cDNA探针杂交。与正常组织相比，5例肾母细胞瘤中的1例出现了额外的条带。结果表明，两条染色体之一上的IGF II基因发生了重排。据推测，这种变化导致了IGF II表达升高，这可能是促进肿瘤生长的一个因素。
