Maus Irena, Wibberg Daniel, Stantscheff Robbin, Stolze Yvonne, Blom Jochen, Eikmeyer Felix-Gregor, Fracowiak Jochen, König Helmut, Pühler Alfred, Schlüter Andreas
Center for Biotechnology, Bielefeld University, Bielefeld, Germany.
Institute of Microbiology and Wine Research, Johannes Gutenberg University Mainz, Mainz, Germany.
J Biotechnol. 2015 May 10;201:43-53. doi: 10.1016/j.jbiotec.2014.11.020. Epub 2014 Nov 29.
The final step of the biogas production process, the methanogenesis, is frequently dominated by members of the genus Methanoculleus. In particular, the species Methanoculleus bourgensis was identified to play a role in different biogas reactor systems. The genome of the type strain M. bourgensis MS2(T), originally isolated from a sewage sludge digestor, was completely sequenced to analyze putative adaptive genome features conferring competitiveness within biogas reactor environments to the strain. Sequencing and assembly of the M. bourgensis MS2(T) genome yielded a chromosome with a size of 2,789,773 bp. Comparative analysis of M. bourgensis MS2(T) and Methanoculleus marisnigri JR1 revealed significant similarities. The absence of genes for a putative ammonium uptake system may indicate that M. bourgensis MS2(T) is adapted to environments rich in ammonium/ammonia. Specific genes featuring predicted functions in the context of osmolyte production were detected in the genome of M. bourgensis MS2(T). Mapping of metagenome sequences derived from a production-scale biogas plant revealed that M. bourgensis MS2(T) almost completely comprises the genetic information of dominant methanogens present in the biogas reactor analyzed. Hence, availability of the M. bourgensis MS2(T) genome sequence may be valuable regarding further research addressing the performance of Methanoculleus species in agricultural biogas plants.
沼气生产过程的最后一步，即产甲烷作用，通常由甲烷袋状菌属的成员主导。特别是，已确定布氏甲烷袋状菌在不同的沼气反应器系统中发挥作用。最初从污水污泥消化器中分离出的模式菌株布氏甲烷袋状菌MS2(T)的基因组被完全测序，以分析赋予该菌株在沼气反应器环境中竞争力的假定适应性基因组特征。布氏甲烷袋状菌MS2(T)基因组的测序和组装产生了一个大小为2,789,773 bp的染色体。布氏甲烷袋状菌MS2(T)与黑海甲烷袋状菌JR1的比较分析显示出显著的相似性。假定的铵摄取系统基因的缺失可能表明布氏甲烷袋状菌MS2(T)适应富含铵/氨的环境。在布氏甲烷袋状菌MS2(T)的基因组中检测到在渗透溶质产生方面具有预测功能的特定基因。对来自生产规模沼气厂的宏基因组序列进行定位分析表明，布氏甲烷袋状菌MS2(T)几乎完全包含了所分析的沼气反应器中优势产甲烷菌的遗传信息。因此，布氏甲烷袋状菌MS2(T)基因组序列的可用性对于进一步研究甲烷袋状菌属在农业沼气厂中的性能可能具有重要价值。
