Tejerizo Gonzalo Torres, Kim Yong Sung, Maus Irena, Wibberg Daniel, Winkler Anika, Off Sandra, Pühler Alfred, Scherer Paul, Schlüter Andreas
Center for Biotechnology (CeBiTec), Bielefeld University, Universitätsstrasse 27, 33615 Bielefeld, Germany; IBBM (Instituto de Biotecnología y Biología Molecular), CCT-CONICET-La Plata, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115, 1900 La Plata, Argentina.
University of Applied Sciences (HAW), Faculty Life Sciences/Research Center "Biomass Utilization Hamburg", Laboratory for Applied Microbiology, Ulmenliet 20, 21033 Hamburg-Bergedorf, Germany.
J Biotechnol. 2017 Apr 10;247:1-5. doi: 10.1016/j.jbiotec.2017.02.015. Epub 2017 Feb 16.
Methanogenic Archaea are of importance at the end of the anaerobic digestion (AD) chain for biomass conversion. They finally produce methane, the end-product of AD. Among this group of microorganisms, members of the genus Methanobacterium are ubiquitously present in anaerobic habitats, such as bioreactors. The genome of a novel methanogenic archaeon, namely Methanobacterium congolense Buetzberg, originally isolated from a mesophilic biogas plant, was completely sequenced to analyze putative adaptive genome features conferring competitiveness of this isolate within the biogas reactor environment. Sequencing and assembly of the M. congolense Buetzberg genome yielded a chromosome with a size of 2,451,457bp and a mean GC-content of 38.51%. Additionally, a plasmid with a size of 18,118bp, featuring a GC content of 36.05% was identified. The M. congolense Buetzberg plasmid showed no sequence similarities with the plasmids described previously suggesting that it represents a new plasmid type. Analysis of the M. congolense Buetzberg chromosome architecture revealed a high collinearity with the Methanobacterium paludis chromosome. Furthermore, annotation of the genome and functional predictions disclosed several genes involved in cell wall and membrane biogenesis. Compilation of specific genes among Methanobacterium strains originating from AD environments revealed 474 genetic determinants that could be crucial for adaptation of these strains to specific conditions prevailing in AD habitats.
产甲烷古菌在生物质转化的厌氧消化（AD）链末端具有重要作用。它们最终产生甲烷，即AD的终产物。在这组微生物中，甲烷杆菌属的成员普遍存在于厌氧生境中，如生物反应器。一种新的产甲烷古菌，即最初从嗜温沼气厂分离出的刚果甲烷杆菌布茨贝格，其基因组已被完全测序，以分析赋予该分离株在沼气反应器环境中竞争力的假定适应性基因组特征。刚果甲烷杆菌布茨贝格基因组的测序和组装产生了一条大小为2,451,457bp、平均GC含量为38.51%的染色体。此外，还鉴定出一个大小为18,118bp、GC含量为36.05%的质粒。刚果甲烷杆菌布茨贝格质粒与先前描述的质粒没有序列相似性，这表明它代表一种新的质粒类型。对刚果甲烷杆菌布茨贝格染色体结构的分析显示，它与沼泽甲烷杆菌染色体具有高度共线性。此外，基因组注释和功能预测揭示了几个参与细胞壁和膜生物合成的基因。对源自AD环境的甲烷杆菌菌株中的特定基因进行汇编后发现，有474个遗传决定因素可能对这些菌株适应AD生境中普遍存在的特定条件至关重要。
