Bock I, Raveh-Amit H, Losonczi E, Carstea A C, Feher A, Mashayekhi K, Matyas S, Dinnyes A, Pribenszky C
Molecular Animal Biotechnology Laboratory, Szent Istvan University, Pater Karoly u. 1. 2100 Godollo, Hungary.
Biotalentum Ltd., Aulich Lajos u. 26. 2100 Godollo, Hungary.
Reprod Fertil Dev. 2016 Apr;28(6):776-84. doi: 10.1071/RD14346.
The efficiency of various assisted reproductive techniques can be improved by preconditioning the gametes and embryos with sublethal hydrostatic pressure treatment. However, the underlying molecular mechanism responsible for this protective effect remains unknown and requires further investigation. Here, we studied the effect of optimised hydrostatic pressure treatment on the global gene expression of mouse oocytes after embryonic genome activation. Based on a gene expression microarray analysis, a significant effect of treatment was observed in 4-cell embryos derived from treated oocytes, revealing a transcriptional footprint of hydrostatic pressure-affected genes. Functional analysis identified numerous genes involved in protein synthesis that were downregulated in 4-cell embryos in response to hydrostatic pressure treatment, suggesting that regulation of translation has a major role in optimised hydrostatic pressure-induced stress tolerance. We present a comprehensive microarray analysis and further delineate a potential mechanism responsible for the protective effect of hydrostatic pressure treatment.
