McCartney M D, Wood T O, McLaughlin B J
Department of Ophthalmology and Visual Sciences, University of Louisville School of Medicine, Kentucky Lions Eye Research Institute, Louisville 40202.
Invest Ophthalmol Vis Sci. 1989 Jul;30(7):1560-4.
The Na+, K+-ATPase pump site density on corneal endothelial cells from Fuchs' endothelial dystrophy corneas has been shown to be drastically decreased in end-stage disease (McCartney et al, Invest Ophthalmol Vis Sci 28:1955, 1987) and significantly increased in the early stages (Geroski et al, Ophthalmology 92:759, 1985) as compared to normal endothelium. In order to provide values for corneas between these two extremes, eye bank corneas from donors with no evidence of corneal edema but with guttata across the extent of the cornea were processed for autoradiography as well as immunohistochemistry. Pump site density was increased compared to end-stage disease but was less than values reported for either functional tissue or early stage disease. Similarly, immunohistochemistry results showed the amount of Na+, K+-ATPase antibody localization to be increased in respect to end-stage disease, but reduced as compared to functional tissue. These results suggest that pump site density on endothelial cells affected with Fuchs' endothelial dystrophy follows a gradual decline towards end-stage disease values as opposed to a sudden sharp deterioration after an initial increase.
与正常内皮相比，在终末期疾病中，富克斯内皮营养不良角膜的角膜内皮细胞上钠钾ATP酶泵位点密度显著降低（McCartney等人，《Invest Ophthalmol Vis Sci》28:1955, 1987），而在早期则显著增加（Geroski等人，《Ophthalmology》92:759, 1985）。为了得出处于这两种极端情况之间的角膜的数值，对来自无角膜水肿迹象但整个角膜有角膜小滴的供体的眼库角膜进行放射自显影和免疫组织化学处理。与终末期疾病相比，泵位点密度增加，但低于功能性组织或早期疾病报告的值。同样，免疫组织化学结果显示，相对于终末期疾病，钠钾ATP酶抗体定位量增加，但与功能性组织相比减少。这些结果表明，受富克斯内皮营养不良影响的内皮细胞上的泵位点密度朝着终末期疾病值逐渐下降，而不是在最初增加后突然急剧恶化。
