Li Xiao-Da, Liang Xiao-Long, Ma Fang, Jing Li-Jia, Lin Li, Yang Yong-Bo, Feng Shan-Shan, Fu Guang-Lei, Yue Xiu-Li, Dai Zhi-Fei
State Key Laboratory of Urban Water Resources and Environment, School of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, PR China.
Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, PR China.
Colloids Surf B Biointerfaces. 2014 Nov 1;123:629-38. doi: 10.1016/j.colsurfb.2014.10.001. Epub 2014 Oct 8.
The lack of biosafety and insufficient delivery efficiency of gene-carriers are still obstacles to human gene therapy. This paper reported highly biocompatible chitosan (CS) functionalized Prussian blue (PB) nanoparticles (designated as CS/PB NPs) for photocontrollable gene delivery. The ultra-small size (∼3 nm), positive charge and high physiological stability of CS/PB NPs make it suitable to be a nonviral vector. In addition, CS/PB NPs could effectively convert the near infrared (NIR) light into heat due to its strong absorption in the NIR region, assisting the uptake of NPs by cells. Upon NIR light irradiation, CS/PB NPs showed superior gene transfection efficiency, much higher than that of free polyethylenimine (PEI). Both in vitro and in vivo experiments demonstrated that CS/PB NPs had excellent biocompatiblity. This work also encourages further exploration of the CS/PB NPs as a photocontrollable nanovector for combined photothermal and gene therapy.
基因载体的生物安全性不足和递送效率低下仍然是人类基因治疗的障碍。本文报道了用于光控基因递送的具有高度生物相容性的壳聚糖（CS）功能化普鲁士蓝（PB）纳米颗粒（命名为CS/PB NPs）。CS/PB NPs的超小尺寸（约3 nm）、正电荷和高生理稳定性使其适合作为非病毒载体。此外，CS/PB NPs由于在近红外（NIR）区域有强烈吸收，能够有效地将近红外光转化为热量，辅助细胞摄取纳米颗粒。在近红外光照射下，CS/PB NPs表现出优异的基因转染效率，远高于游离聚乙烯亚胺（PEI）。体外和体内实验均表明CS/PB NPs具有优异的生物相容性。这项工作也鼓励进一步探索将CS/PB NPs作为用于光热和基因联合治疗的光控纳米载体。
