Department of Biochemistry and Molecular Biology, Federal University of Paraná, P.B. 19046, Centro Politécnico, CEP 81531-990 Curitiba, PR, Brazil.
Department of Chemistry, Federal University of Paraná, P.B. 19046, Centro Politécnico, CEP 81531-990 Curitiba, PR, Brazil.
Carbohydr Polym. 2015 Feb 13;116:229-36. doi: 10.1016/j.carbpol.2014.07.043. Epub 2014 Jul 29.
The curcumin (CUR)-loaded binary hydrogel was formulated using xanthan and galactomannan from Schizolobium parahybae (guapuruvu). The binary hydrogels presented gel characteristics, stable pH values and mechanical stress resistance even after 45 days of heat exposure (45 °C). The CUR-loaded hydrogel content was 98.6% for XGMC (xanthan and galactomannan with CUR-microemulsion) after the stability test. The in vitro cytotoxicity analysis suggested non-cutaneous membrane irritation, and the in vitro skin permeation analysis indicated 2.15 to 2.50 μg mL(-1) CUR at the stratum corneum, epidermal and dermal levels. The XGEC (xanthan and galactomannan with CUR solubilized in ethanol) and XGMC hydrogels presented 76.8 and 63.2% inhibition of topical inflammation, respectively. Chemical stability and non-cytotoxicity analysis confirm the safety of prolonged exposure of the skin during the topical treatment, offering long-lasting XGEC and XGMC action.
姜黄素（CUR）负载的二元水凝胶是使用来自 Schizolobium parahybae（guapuruvu）的黄原胶和半乳甘露聚糖制备的。二元水凝胶具有凝胶特性，即使在 45°C 下暴露 45 天，其 pH 值和机械应力稳定性仍保持稳定。经过稳定性测试后，XGMC（载有 CUR-微乳液的黄原胶和半乳甘露聚糖）中 CUR 负载水凝胶的含量为 98.6%。体外细胞毒性分析表明无皮肤刺激性，体外皮肤渗透分析表明在角质层、表皮和真皮水平有 2.15 至 2.50μgmL（-1）CUR。XGEC（乙醇中溶解 CUR 的黄原胶和半乳甘露聚糖）和 XGMC 水凝胶分别对局部炎症有 76.8%和 63.2%的抑制作用。化学稳定性和非细胞毒性分析证实了皮肤在局部治疗期间长时间暴露的安全性，为 XGEC 和 XGMC 的长效作用提供了保障。
