Sanches Eduardo Antônio, Okawara Renan Yoshiharu, Caneppele Danilo, Neumann Giovano, Bombardelli Robie Allan, Romagosa Elizabeth
State University of São Paulo, UNESP, Registro, SP, Brazil.
Fishery Institute, APTA, São Paulo, SP, Brazil.
Anim Reprod Sci. 2014 Dec 30;151(3-4):262-8. doi: 10.1016/j.anireprosci.2014.09.022. Epub 2014 Oct 14.
The objective of this study was to assess the influence of temperature and time on the storage of fresh Steindachneridion parahybae oocytes. Two experiments were carried out: (1) the fertilization rates of oocytes exposed to temperatures of 5, 15, 28 (room temperature) and 35°C were assessed 15min (control), 115, 235 and 355min after release; (2) the fertilization and hatching rates, as well as the percentage of normal larvae of oocytes exposed to 14, 17 or 20°C, 20min (control) were assessed 50, 80 and 110min after stripping. In the first experiment, the highest fertilization rates (P<0.05) were obtained in the control treatment (15min, 28°C), with 74.34±5.48% oocytes showing loss of viability over time. In the second experiment, there was a reduction (P<0.05) in the fertilization rates at the temperatures and times tested. The artificial fertilization of S. parahybae oocytes is recommended immediately after collection, and if storage is necessary, it should be conducted at temperatures between 17 and 20°C.
本研究的目的是评估温度和时间对淡水魟（Steindachneridion parahybae）新鲜卵母细胞储存的影响。进行了两项实验：（1）在卵母细胞释放后15分钟（对照）、115、235和355分钟，评估暴露于5、15、28（室温）和35°C温度下的卵母细胞的受精率；（2）在采卵后50、80和110分钟，评估暴露于14、17或20°C、20分钟（对照）的卵母细胞的受精率、孵化率以及正常仔鱼的百分比。在第一个实验中，对照处理（15分钟，28°C）获得了最高的受精率（P<0.05），随着时间的推移，有74.34±5.48%的卵母细胞丧失活力。在第二个实验中，在所测试的温度和时间下，受精率有所降低（P<0.05）。建议淡水魟卵母细胞采集后立即进行人工授精，如需储存，应在17至20°C的温度下进行。
