Angrimani D S R, Lucio C F, Veiga G A L, Silva L C G, Regazzi F M, Nichi M, Vannucchi C I
Department of Animal Reproduction, Faculty of Veterinary Medicine, University of São Paulo, São Paulo, Brazil.
Andrologia. 2014 Sep;46(7):814-9. doi: 10.1111/and.12154. Epub 2013 Aug 22.
Spermatozoa become more susceptible to the attack of reactive oxygen species during maturation. To avoid oxidative damage, the epididymis must provide the necessary antioxidant protection. The aim of this study was to compare the canine sperm profile and the enzymatic antioxidant status of the ejaculated fractions and samples collected from the different segments of the epididymis (caput, corpus and cauda). Five adult dogs were used, and after 1-3 weeks, subsequently to bilateral orchiectomy and epididymal storage, sperm samples were collected from the different segments of the epididymis. Samples were evaluated for conventional microscopy and computer-assisted motility analysis: sperm plasma membrane permeability and the activity of the antioxidant enzymes catalase, glutathione peroxidase (GPx) and superoxide dismutase (SOD). Samples collected from the caput and corpus showed lower values for most of the motility variables evaluated, indicating different levels of immaturity. Catalase activity was observed only in ejaculated samples. Conversely, GPx activity was higher in the cauda epididymidis. Correlations were found between SOD and GPx and SOD and sperm motility in the epididymal cauda and corpus, highlighting the importance of the enzymes for the protection of spermatozoa during the transit along the epididymis.
精子在成熟过程中对活性氧的攻击变得更加敏感。为避免氧化损伤，附睾必须提供必要的抗氧化保护。本研究的目的是比较犬的精子特征以及从附睾不同节段（头部、体部和尾部）采集的射精部分和样本的酶促抗氧化状态。使用了5只成年犬，在双侧睾丸切除和附睾储存1 - 3周后，从附睾的不同节段采集精子样本。对样本进行常规显微镜检查和计算机辅助运动分析：评估精子质膜通透性以及抗氧化酶过氧化氢酶、谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）的活性。从附睾头部和体部采集的样本在大多数评估的运动变量方面显示出较低的值，表明不同程度的不成熟。仅在射精样本中观察到过氧化氢酶活性。相反，附睾尾部的GPx活性较高。在附睾尾部和体部发现SOD与GPx以及SOD与精子活力之间存在相关性，突出了这些酶在精子沿附睾转运过程中对精子保护的重要性。
