小鼠逆转录病毒长末端重复序列增强子功能区的图谱绘制：增强子结构域相互作用，且在对增强子活性的贡献中并非独立发挥作用。
Mapping of functional regions of murine retrovirus long terminal repeat enhancers: enhancer domains interact and are not independent in their contributions to enhancer activity.
作者信息
Hollon T, Yoshimura F K
机构信息
Department of Microbiology, University of Washington, Seattle 98195.
出版信息
J Virol. 1989 Aug;63(8):3353-61. doi: 10.1128/JVI.63.8.3353-3361.1989.
Abstract
We have used deletion and recombinant long terminal repeat (LTR) mutants to examine enhancer activity differences between LTRs of the nonpathogenic Akv and the thymus lymphomagenic MCF13 murine retroviruses. Deletion mutant analysis revealed that major control regions for MCF13 and Akv LTR enhancer activity were similar but not identical. For both LTRs, major control regions were distinctly different in a murine T-cell and a fibroblast cell line. Recombinant enhancer analysis showed that LTRs could be divided into three regions capable of altering the level of enhancer activity through cooperative or antagonistic interaction. The contribution of each region to enhancer activity was dependent on its context with respect to the other regions. LTR enhancer function in different cell types appears to be the result of the interaction of enhancer modular elements.
摘要
我们利用缺失和重组长末端重复序列（LTR）突变体来检测非致病性Akv和胸腺淋巴瘤致瘤性MCF13鼠逆转录病毒的LTR之间的增强子活性差异。缺失突变体分析表明，MCF13和Akv LTR增强子活性的主要控制区域相似但并不相同。对于这两种LTR，在鼠T细胞和成纤维细胞系中，主要控制区域明显不同。重组增强子分析表明，LTR可分为三个区域，这些区域能够通过协同或拮抗相互作用改变增强子活性水平。每个区域对增强子活性的贡献取决于其相对于其他区域的背景。不同细胞类型中LTR增强子功能似乎是增强子模块元件相互作用的结果。
相似文献
1
Mapping of functional regions of murine retrovirus long terminal repeat enhancers: enhancer domains interact and are not independent in their contributions to enhancer activity.小鼠逆转录病毒长末端重复序列增强子功能区的图谱绘制：增强子结构域相互作用，且在对增强子活性的贡献中并非独立发挥作用。
J Virol. 1989 Aug;63(8):3353-61. doi: 10.1128/JVI.63.8.3353-3361.1989.
2
Differential DNA binding of nuclear proteins to a long terminal repeat region of the MCF13 and Akv murine leukemia viruses.核蛋白与MCF13和Akv鼠白血病病毒长末端重复序列区域的差异DNA结合
J Virol. 1989 Nov;63(11):4945-8. doi: 10.1128/JVI.63.11.4945-4948.1989.
3
Contributions to transcriptional activity and to viral leukemogenicity made by sequences within and downstream of the MCF13 murine leukemia virus enhancer.MCF13鼠白血病病毒增强子内部及下游序列对转录活性和病毒致白血病性的作用。
J Virol. 1992 Dec;66(12):7080-8. doi: 10.1128/JVI.66.12.7080-7088.1992.
4
Negative regulatory element associated with potentially functional promoter and enhancer elements in the long terminal repeats of endogenous murine leukemia virus-related proviral sequences.与内源性鼠白血病病毒相关前病毒序列长末端重复序列中潜在功能性启动子和增强子元件相关的负调控元件。
J Virol. 1989 Jun;63(6):2746-57. doi: 10.1128/JVI.63.6.2746-2757.1989.
5
Sequences responsible for erythroid and lymphoid leukemia in the long terminal repeats of Friend-mink cell focus-forming and Moloney murine leukemia viruses.弗瑞德-貂细胞集落形成病毒和莫洛尼鼠白血病病毒长末端重复序列中负责红系和淋巴系白血病的序列。
J Virol. 1987 Jun;61(6):1861-6. doi: 10.1128/JVI.61.6.1861-1866.1987.
6
Identification of a region of a murine leukemia virus long terminal repeat with novel transcriptional regulatory activities.鉴定具有新型转录调控活性的鼠白血病病毒长末端重复序列区域。
J Virol. 1994 May;68(5):3308-16. doi: 10.1128/JVI.68.5.3308-3316.1994.
7
Role of the LTR region between the enhancer and promoter in mink cell focus-forming murine leukemia virus pathogenesis.增强子与启动子之间的长末端重复序列（LTR）区域在貂细胞灶形成性鼠白血病病毒致病机制中的作用。
Virology. 2001 Apr 25;283(1):121-31. doi: 10.1006/viro.2001.0879.
8
B-Cell lymphoma induction by akv murine leukemia viruses harboring one or both copies of the tandem repeat in the U3 enhancer.携带U3增强子中串联重复序列一个或两个拷贝的Akv鼠白血病病毒诱导B细胞淋巴瘤
J Virol. 1998 Jul;72(7):5745-56. doi: 10.1128/JVI.72.7.5745-5756.1998.
9
The malignant histiocytosis sarcoma virus, a recombinant of Harvey murine sarcoma virus and Friend mink cell focus-forming virus, has acquired myeloid transformation specificity by alterations in the long terminal repeat.恶性组织细胞增多症肉瘤病毒，是哈维鼠肉瘤病毒和弗瑞德貂细胞集落形成病毒的重组体，通过长末端重复序列的改变获得了髓系转化特异性。
J Virol. 1990 Jan;64(1):369-78. doi: 10.1128/JVI.64.1.369-378.1990.
10
Analysis of the significance of two single-base-pair differences in the SL3-3 and Akv virus long terminal repeats.SL3-3和Akv病毒长末端重复序列中两个单碱基对差异的意义分析。
J Virol. 1991 Feb;65(2):1019-22. doi: 10.1128/JVI.65.2.1019-1022.1991.
引用本文的文献
1
Duplication of U3 sequences in the long terminal repeat of mink cell focus-inducing viruses generates redundancies of transcription factor binding sites important for the induction of thymomas.水貂细胞融合诱导病毒长末端重复序列中U3序列的重复产生了对胸腺瘤诱导至关重要的转录因子结合位点冗余。
J Virol. 2003 Mar;77(5):3326-33. doi: 10.1128/jvi.77.5.3326-3333.2003.
2
Sequence analysis of porcine endogenous retrovirus long terminal repeats and identification of transcriptional regulatory regions.猪内源性逆转录病毒长末端重复序列的序列分析及转录调控区域的鉴定
J Virol. 2003 Jan;77(1):142-9. doi: 10.1128/jvi.77.1.142-149.2003.
3
Ikaros, a lymphoid-cell-specific transcription factor, contributes to the leukemogenic phenotype of a mink cell focus-inducing murine leukemia virus.伊卡洛斯是一种淋巴细胞特异性转录因子，它对水貂细胞灶诱导型鼠白血病病毒的致白血病表型有影响。
J Virol. 2002 Jan;76(1):78-87. doi: 10.1128/jvi.76.1.78-87.2002.
4
Members of the GATA family of transcription factors bind to the U3 region of Cas-Br-E and graffi retroviruses and transactivate their expression.转录因子GATA家族的成员与Cas-Br-E和格拉菲逆转录病毒的U3区域结合，并激活它们的表达。
J Virol. 1998 Jul;72(7):5579-88. doi: 10.1128/JVI.72.7.5579-5588.1998.
5
Analysis of enhancer function of the HS-40 core sequence of the human alpha-globin cluster.人α-珠蛋白基因簇HS-40核心序列增强子功能分析
Nucleic Acids Res. 1997 Jul 15;25(14):2917-22. doi: 10.1093/nar/25.14.2917.
6
A protein-binding site with dyad symmetry in the long terminal repeat of the MCF13 murine leukemia virus that contributes to transcriptional activity in T lymphocytes.MCF13鼠白血病病毒长末端重复序列中具有二元对称的蛋白质结合位点，其有助于T淋巴细胞中的转录活性。
J Virol. 1993 Apr;67(4):2298-304. doi: 10.1128/JVI.67.4.2298-2304.1993.
7
Identification of a region of a murine leukemia virus long terminal repeat with novel transcriptional regulatory activities.鉴定具有新型转录调控活性的鼠白血病病毒长末端重复序列区域。
J Virol. 1994 May;68(5):3308-16. doi: 10.1128/JVI.68.5.3308-3316.1994.
8
Pathogenic determinants in the U3 region of recombinant murine leukemia viruses isolated from CWD and HRS/J mice.从慢性消耗病（CWD）和HRS/J小鼠中分离出的重组鼠白血病病毒U3区域的致病决定因素。
J Virol. 1994 Aug;68(8):5174-83. doi: 10.1128/JVI.68.8.5174-5183.1994.
9
Amplified and tissue-directed expression of retroviral vectors using ping-pong techniques.利用乒乓技术实现逆转录病毒载体的扩增及组织定向表达。
J Mol Med (Berl). 1995 Mar;73(3):113-20. doi: 10.1007/BF00198238.
10
Differential DNA binding of nuclear proteins to a long terminal repeat region of the MCF13 and Akv murine leukemia viruses.核蛋白与MCF13和Akv鼠白血病病毒长末端重复序列区域的差异DNA结合
J Virol. 1989 Nov;63(11):4945-8. doi: 10.1128/JVI.63.11.4945-4948.1989.
本文引用的文献
1
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.在哺乳动物细胞中表达氯霉素乙酰转移酶的重组基因组。
Mol Cell Biol. 1982 Sep;2(9):1044-51. doi: 10.1128/mcb.2.9.1044-1051.1982.
2
Characterization of enhancer elements in the long terminal repeat of Moloney murine sarcoma virus.莫洛尼鼠肉瘤病毒长末端重复序列中增强子元件的特性分析。
J Virol. 1984 Jan;49(1):183-9. doi: 10.1128/JVI.49.1.183-189.1984.
3
Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation.通过电穿孔导入小鼠前B淋巴细胞的人κ免疫球蛋白基因的增强子依赖性表达。
Proc Natl Acad Sci U S A. 1984 Nov;81(22):7161-5. doi: 10.1073/pnas.81.22.7161.
4
Determination of the leukaemogenicity of a murine retrovirus by sequences within the long terminal repeat.通过长末端重复序列内的序列测定鼠逆转录病毒的致白血病性。
Nature. 1984;308(5958):467-70. doi: 10.1038/308467a0.
5
Thymotropism of murine leukemia virus is conferred by its long terminal repeat.鼠白血病病毒的亲胸腺性由其长末端重复序列赋予。
Proc Natl Acad Sci U S A. 1983 Jul;80(14):4203-7. doi: 10.1073/pnas.80.14.4203.
6
Enhancer elements.增强子元件
Cell. 1983 Jun;33(2):313-4. doi: 10.1016/0092-8674(83)90410-5.
7
Expression and regulation of Escherichia coli lacZ gene fusions in mammalian cells.大肠杆菌lacZ基因融合体在哺乳动物细胞中的表达与调控
J Mol Appl Genet. 1983;2(1):101-9.
8
Host-specific activation of transcription by tandem repeats from simian virus 40 and Moloney murine sarcoma virus.来自猴病毒40和莫洛尼氏鼠肉瘤病毒的串联重复序列对转录的宿主特异性激活。
Proc Natl Acad Sci U S A. 1982 Nov;79(21):6453-7. doi: 10.1073/pnas.79.21.6453.
9
Long terminal repeat of murine retroviral DNAs: sequence analysis, host-proviral junctions, and preintegration site.小鼠逆转录病毒DNA的长末端重复序列：序列分析、宿主-病毒连接及整合前位点
J Virol. 1982 Feb;41(2):542-56. doi: 10.1128/JVI.41.2.542-556.1982.
10
Adenovirus DNA replication in vitro: identification of a host factor that stimulates synthesis of the preterminal protein-dCMP complex.腺病毒DNA的体外复制：一种刺激末端前蛋白-dCMP复合物合成的宿主因子的鉴定。
Proc Natl Acad Sci U S A. 1982 Nov;79(21):6438-42. doi: 10.1073/pnas.79.21.6438.
Zotero 插件