Van Beveren C, Rands E, Chattopadhyay S K, Lowy D R, Verma I M
J Virol. 1982 Feb;41(2):542-56. doi: 10.1128/JVI.41.2.542-556.1982.
The nucleotide sequence of the long terminal repeat (LTR) of three murine retroviral DNAs has been determined. The data indicate that the U5 region (sequences originating from the 5' end of the genome) of various LTRs is more conserved than the U3 region (sequences from the 3' end of the genome). The location and sequence of the control elements such as the 5' cap, "TATA-like" sequences, "CCAAT-box," and presumptive polyadenylic acid addition signal AATAAA in the various LTRs are nearly identical. Some murine retroviral DNAs contain a duplication of sequences within the LTR ranging in size from 58 to 100 base pairs. A variant of molecularly cloned Moloney murine sarcoma virus DNA in which one of the two LTRs integrated into the viral DNA was also analyzed. A 4-base-pair duplication was generated at the site of integration of LTR in the viral DNA. The host-viral junction of two molecularly cloned AKR-murine leukemia virus DNAs (clones 623 and 614) was determined. In the case of AKR-623 DNA, a 3- or 4-base-pair direct repeat of cellular sequences flanking the viral DNA was observed. However, AKR-614 DNA contained a 5-base-pair repeat of cellular sequences. The nucleotide sequence of the preintegration site of AKR-623 DNA revealed that the cellular sequences duplicated during integration are present only once. Finally, a striking homology between the sequences flanking the preintegration site and viral LTRs was observed.
已确定三种鼠逆转录病毒DNA长末端重复序列（LTR）的核苷酸序列。数据表明，各种LTR的U5区域（源自基因组5'端的序列）比U3区域（源自基因组3'端的序列）更保守。各种LTR中控制元件的位置和序列，如5'帽、“类TATA”序列、“CCAAT盒”以及推测的聚腺苷酸添加信号AATAAA几乎相同。一些鼠逆转录病毒DNA在LTR内含有大小从58到100个碱基对不等的序列重复。还分析了分子克隆的莫洛尼鼠肉瘤病毒DNA的一个变体，其中整合到病毒DNA中的两个LTR之一。在病毒DNA中LTR的整合位点产生了一个4个碱基对的重复。确定了两个分子克隆的AKR-鼠白血病病毒DNA（克隆623和614）的宿主-病毒连接点。在AKR-623 DNA的情况下，观察到病毒DNA两侧细胞序列的3或4个碱基对直接重复。然而，AKR-614 DNA含有细胞序列的5个碱基对重复。AKR-623 DNA整合前位点的核苷酸序列显示，整合过程中重复的细胞序列仅出现一次。最后，观察到整合前位点两侧序列与病毒LTR之间存在显著同源性。
