Kimura Y, Okuda H
Second Department of Medical Biochemistry, School of Medicine, Ehime University, Japan.
J Ethnopharmacol. 1989 May;25(3):269-80. doi: 10.1016/0378-8741(89)90033-0.
It was found that the EtO Ac fraction of the roots of Angelica shikokiana inhibited adrenaline-induced lipolysis in rat fat cells, while having no effect on ACTH-induced lipolysis. On the other hand, the MeOH fraction was found to inhibit ACTH-induced lipolysis but not to inhibit adrenaline-induced lipolysis. The active substances isolated from this root were elucidated to be psoralen (I), bergapten (II), isopteryxin(3'-angeloyloxy-4'-acetoxy-3',4'-dihydroseseli n) (III) and 3'-epoxyangeloyloxy-4'-acetoxy-3',4'-dihydroseselin (IV), respectively. The two furocoumarins, psoralen and bergapten, were found to enhance ACTH- and adrenaline-induced lipolysis in fat cells respectively. In contrast, a coumarin derivative of khellactone type, isopteryxin (III), was found to inhibit the adrenaline-induced lipolysis but with no effect on ACTH-induced lipolysis and insulin-induced lipogenesis from glucose. The other coumarin of khellactone type, 3'(R), 4'(R)-3'-epoxyangeloyloxy-4'-acetoxy-3',4'-dihydroseseline (IV), was found to inhibit the insulin-induced lipogenesis from glucose but it had no effect on adrenaline- and ACTH-induced lipolysis.
结果发现，日本当归根的乙酸乙酯部位可抑制大鼠脂肪细胞中肾上腺素诱导的脂肪分解，而对促肾上腺皮质激素（ACTH）诱导的脂肪分解无影响。另一方面，甲醇部位可抑制ACTH诱导的脂肪分解，但不抑制肾上腺素诱导的脂肪分解。从该根中分离出的活性物质分别鉴定为补骨脂素（I）、佛手柑内酯（II）、异翅芹素（3'-当归酰氧基-4'-乙酰氧基-3',4'-二氢升麻素）（III）和3'-环氧当归酰氧基-4'-乙酰氧基-3',4'-二氢升麻素（IV）。发现两种呋喃香豆素，即补骨脂素和佛手柑内酯，分别增强脂肪细胞中ACTH和肾上腺素诱导的脂肪分解。相比之下，一种凯刺酮型香豆素衍生物异翅芹素（III）可抑制肾上腺素诱导的脂肪分解，但对ACTH诱导的脂肪分解和胰岛素诱导的葡萄糖脂肪生成无影响。另一种凯刺酮型香豆素，3'(R), 4'(R)-3'-环氧当归酰氧基-4'-乙酰氧基-3',4'-二氢升麻素（IV），可抑制胰岛素诱导的葡萄糖脂肪生成，但对肾上腺素和ACTH诱导的脂肪分解无影响。
