Post Graduate Department of Microbiology, Biogas Research and Extension Centre, Gujarat Vidyapith, Sadra 382 320, Gujarat, India.
Post Graduate Department of Microbiology, Biogas Research and Extension Centre, Gujarat Vidyapith, Sadra 382 320, Gujarat, India.
Bioresour Technol. 2015 Jan;175:1-7. doi: 10.1016/j.biortech.2014.10.040. Epub 2014 Oct 20.
The present research emphasizes on degradation of azo dyes from simulated textile wastewater using down flow microaerophilic fixed film reactor. Degradation of simulated textile wastewater (COD 7200mg/L and dye concentration 300mg/L) was studied in a microaerophilic fixed film reactor using pumice stone as a support material under varying hydraulic retention time (HRT) and organic loading rate (OLR). The intense metabolic activity of the inoculated bacterial consortium in the reactor led to 97.5% COD reduction and 99.5% decolorization of simulated wastewater operated under OLR of 7.2kgCODm(3)/d and 24h of HRT. FTIR, (1)H NMR and GC-MS studies revealed the formation of lower molecular weight aliphatic compounds under 24h of HRT, leading to complete mineralization of simulated wastewater. The detection of oxido-reductive enzyme activities suggested the enzymatic reduction of azo bonds prior to mineralization. Toxicity studies indicated that microbial treatment favors detoxification of simulated wastewater.
本研究强调使用下流式微好氧固定膜反应器从模拟纺织废水中降解偶氮染料。在微好氧固定膜反应器中，以浮石为支撑材料，在不同水力停留时间（HRT）和有机负荷率（OLR）下，研究了模拟纺织废水（COD7200mg/L 和染料浓度 300mg/L）的降解情况。接种细菌混合物在反应器中的强烈代谢活动导致模拟废水在 OLR 为 7.2kgCODm(3)/d 和 HRT 为 24 小时的条件下，COD 去除率达到 97.5%，脱色率达到 99.5%。傅里叶变换红外光谱（FTIR）、（1）H 核磁共振（NMR）和气相色谱-质谱联用（GC-MS）研究表明，在 24 小时的 HRT 下形成了较低分子量的脂肪族化合物，导致模拟废水完全矿化。氧化还原酶活性的检测表明，在矿化之前，偶氮键通过酶促还原进行了降解。毒性研究表明，微生物处理有利于模拟废水的解毒。
