Saratale Rijuta G, Saratale Ganesh D, Govindwar Sanjay P, Kim Dong S
a Department of Environmental Science and Engineering , Ewha Womans University , Seoul , Republic of Korea.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2015;50(2):176-92. doi: 10.1080/10934529.2014.975536.
Complete decolorization and detoxification of Reactive Orange 4 within 5 h (pH 6.6, at 30°C) by isolated Lysinibacillus sp. RGS was observed. Significant reduction in TOC (93%) and COD (90%) was indicative of conversion of complex dye into simple products, which were identified as naphthalene moieties by various analytical techniques (HPLC, FTIR, and GC-MS). Supplementation of agricultural waste extract considered as better option to make the process cost effective. Oxido-reductive enzymes were found to be involved in the degradation mechanism. Finally Loofa immobilized Lysinibacillus sp. cells in a fixed-bed bioreactor showed significant decolorization with reduction in TOC (51 and 64%) and COD (54 and 66%) for synthetic and textile effluent at 30 and 35 mL h(-1) feeding rate, respectively. The degraded metabolites showed non-toxic nature revealed by phytotoxicity and photosynthetic pigments content study for Sorghum vulgare and Phaseolus mungo. In addition nitrogen fixing and phosphate solubilizing microbes were less affected in treated wastewater and thus the treated effluent can be used for the irrigation purpose. This work could be useful for the development of efficient and ecofriendly technologies to reduce dye content in the wastewater to permissible levels at affordable cost.
在30°C、pH 6.6条件下，分离得到的赖氨酸芽孢杆菌RGS能在5小时内实现活性橙4的完全脱色和解毒。TOC（93%）和COD（90%）的显著降低表明复杂的染料已转化为简单产物，通过各种分析技术（HPLC、FTIR和GC-MS）鉴定这些产物为萘部分。添加农业废弃物提取物被认为是使该过程具有成本效益的更好选择。发现氧化还原酶参与了降解机制。最后，在固定床生物反应器中，丝瓜固定化的赖氨酸芽孢杆菌细胞在进料速率分别为30和35 mL h(-1)时，对合成废水和纺织废水表现出显著的脱色效果，TOC（分别为51%和64%）和COD（分别为54%和66%）降低。通过对高粱和绿豆的植物毒性和光合色素含量研究表明，降解代谢产物具有无毒性质。此外，处理后的废水中固氮和溶磷微生物受影响较小，因此处理后的废水可用于灌溉。这项工作对于开发高效且环保的技术以将废水中的染料含量降低到允许水平并以可承受的成本具有重要意义。
