College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China.
College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China.
Bioresour Technol. 2015 Jan;175:42-50. doi: 10.1016/j.biortech.2014.10.059. Epub 2014 Oct 18.
Focusing on the task-specific properties of ionic liquids (ILs), a novel IL was designed by combining the quaternary ammonium cationic surfactant, N1,1,1,1, with benign amino acid anion (Cys), and was successfully employed in whole-cell-catalyzed bioreduction by Trichodermaasperellum ZJPH0810 using an ionic liquid-distilled water medium. As expected, based on better understanding about the effects of ILs' characteristics of cations and anions on T. asperellum ZJPH0810-catalyzed bioreduction and the optimization of reaction parameters, the developed tetramethylammonium cysteine ([N1,1,1,1][Cys])-containing system is more efficient for the asymmetric reduction of 3,5-bis(trifluoromethyl) acetophenone to (R)-[3,5-bis(trifluoromethyl)phenyl] ethanol by whole-cell catalysis in contrast to that in monophasic aqueous system. Herein, a new biotechnological application for designed type of ILs is proposed due to its novel property of strengthening coenzyme regeneration during the bioreduction process. The designed IL [N1,1,1,1][Cys] was also evaluated in Candidatropicalis 104-catalyzed bioreduction to board its application, with remarkable success.
聚焦于离子液体（ILs）的任务特异性性质，通过将季铵阳离子表面活性剂N1,1,1,1与良性氨基酸阴离子(Cys)结合，设计了一种新型 IL，并成功地用于 Trichoderma asperellum ZJPH0810 全细胞催化的生物还原反应中，使用离子液体-蒸馏水介质。不出所料，基于更好地了解 ILs 的阳离子和阴离子特性对 T. asperellum ZJPH0810 催化生物还原的影响，并对反应参数进行优化，开发的四甲基半胱氨酸([N1,1,1,1][Cys])-含体系在两相均一水溶液体系中更有效地进行不对称还原 3,5-双（三氟甲基）苯乙酮为（R）-[3,5-双（三氟甲基）苯基]乙醇。在此，由于其在生物还原过程中增强辅酶再生的新特性，提出了设计类型的 IL 的一种新的生物技术应用。所设计的 IL [N1,1,1,1][Cys]也在 Candida tropicalis 104 催化的生物还原中进行了评估，以扩大其应用，取得了显著的成功。
