Lee Do Kyung, Park Jae Eun, Kim Min Ji, Seo Jae Goo, Lee Ji Hyuk, Ha Nam Joo
College of Pharmacy, Sahmyook University, Nowon, Seoul 139-742, Republic of Korea.
R&D Center, Cellbiotech, Co. Ltd., Gimpo, Gyeonggi 157-030, Republic of Korea.
Clin Res Hepatol Gastroenterol. 2015 Apr;39(2):237-44. doi: 10.1016/j.clinre.2014.09.006. Epub 2014 Nov 12.
Evidence suggests that specific probiotics may be antagonistic to enteric pathogens and enhance immunity, and thus, provide a means of preventing or treating diarrheal diseases. In the present study, we aimed to evaluate the efficacy of probiotic strains isolated from Koreans for the treatment of viral gastroenteritis in young children and against rotavirus in vitro.
In vitro antiviral activities of probiotic isolates on rotavirus infection were investigated in the Vero cell using a plaque reduction assay. Then several probiotic strains with the high antiviral activity were chosen for further clinical trials. Twenty-nine pediatric patients who presented with symptoms of viral gastroenteritis were enrolled in a double-blind trial and randomly assigned at admission to receive six probiotic strains (Bifidobacterium longum, B. lactis, Lactobacillus acidophilus, L. rhamnosus, L. plantarum, and Pediococcus pentosaceus) at a dose of 10(9) colony forming units/g or a comparable placebo twice daily for 1 week.
Of the tested probiotic strains, B. longum isolated from an infant showed the greatest inhibitory effect and L. acidophilus showed the second-highest inhibitory effect. These probiotics significantly shortened the duration of diarrhea as compared with a placebo (6.1 ± 0.5 vs 7.2 ± 1.9, P = 0.030) and did not induce any adverse effects. Our findings suggest that the probiotic strains selected in the present study may be useful for the treatment of acute rotaviral gastroenteritis or as an alternative therapy without adverse effects.
有证据表明，特定益生菌可能对肠道病原体具有拮抗作用并增强免疫力，从而提供一种预防或治疗腹泻病的方法。在本研究中，我们旨在评估从韩国人分离出的益生菌菌株对幼儿病毒性肠胃炎的治疗效果以及在体外对轮状病毒的作用。
使用蚀斑减少试验在Vero细胞中研究益生菌分离株对轮状病毒感染的体外抗病毒活性。然后选择几种具有高抗病毒活性的益生菌菌株进行进一步的临床试验。29名出现病毒性肠胃炎症状的儿科患者参加了一项双盲试验，并在入院时随机分配，接受六种益生菌菌株（长双歧杆菌、乳酸双歧杆菌、嗜酸乳杆菌、鼠李糖乳杆菌、植物乳杆菌和戊糖片球菌），剂量为10⁹ 菌落形成单位/克，或一种相当的安慰剂，每天两次，持续1周。
在测试的益生菌菌株中，从一名婴儿分离出的长双歧杆菌显示出最大的抑制作用，嗜酸乳杆菌显示出第二高的抑制作用。与安慰剂相比，这些益生菌显著缩短了腹泻持续时间（6.1±0.5天对7.2±1.9天，P = 0.030），并且未引起任何不良反应。我们的研究结果表明，本研究中选择的益生菌菌株可能对治疗急性轮状病毒性肠胃炎有用，或作为一种无不良反应的替代疗法。
