Juntunen M, Kirjavainen P V, Ouwehand A C, Salminen S J, Isolauri E
Department of Pediatrics, Satakunta Central Hospital, Pori, FIN-25800 Pori, Finland.
Clin Diagn Lab Immunol. 2001 Mar;8(2):293-6. doi: 10.1128/CDLI.8.2.293-296.2001.
The concentration of fecal mucin and the adhesion of specific probiotics and their combinations in the intestinal mucus of infants during and after rotavirus diarrhea and in healthy children were determined. Mucus was prepared from fecal samples from 20 infants during and after rotavirus diarrhea and from 10 healthy age-matched children. Mucin concentration was determined, and the adhesion of five probiotics-Lactobacillus rhamnosus GG, Lactobacillus casei Shirota, Lactobacillus paracasei F19, Lactobacillus acidophilus LA5, and Bifidobacterium lactis Bb12-and their combinations was tested in vitro. The mean concentrations of fecal mucin during and after rotavirus diarrhea, 15.2 and 14.1 mg/g, were comparable to that in healthy children, 14.9 mg/g. The adherence of probiotics ranged from 1 to 34% in healthy subjects as indicated for the following strains: L. rhamnosus GG, 34%; B. lactis Bb12, 31%; L. acidophilus LA5, 4%; L. paracasei F19, 3%; and L. casei Shirota, 1% (P = 0.0001). The distinctive pattern of probiotic adherence was not influenced by rotavirus diarrhea. The adhesion of Bb12 in the presence of GG increased from 31 to 39% in healthy infants (P = 0.018) and in episodes of diarrhea increased from 26 to 44% (P = 0.001). Rotavirus diarrhea does not decrease the production of fecal mucin or with respect to the adhesion of probiotic bacteria tested in vitro. Combination of specific probiotic strains may enhance adherence in a synergistic manner. Optimal clinical application of these interactions may offer novel therapeutic guidelines for the treatment and prevention of gastrointestinal infections.
测定了轮状病毒腹泻期间及之后婴儿以及健康儿童肠道黏液中粪便黏蛋白的浓度、特定益生菌及其组合的黏附情况。从20名轮状病毒腹泻期间及之后的婴儿以及10名年龄匹配的健康儿童的粪便样本中制备黏液。测定黏蛋白浓度，并在体外测试了5种益生菌——鼠李糖乳杆菌GG、干酪乳杆菌代田株、副干酪乳杆菌F19、嗜酸乳杆菌LA5和双歧杆菌Bb12——及其组合的黏附情况。轮状病毒腹泻期间及之后粪便黏蛋白的平均浓度分别为15.2和14.1mg/g，与健康儿童的14.9mg/g相当。在健康受试者中，益生菌的黏附率在1%至34%之间，如下列菌株所示：鼠李糖乳杆菌GG为34%；双歧杆菌Bb12为31%；嗜酸乳杆菌LA5为4%；副干酪乳杆菌F19为3%；干酪乳杆菌代田株为1%（P = 0.0001）。益生菌独特的黏附模式不受轮状病毒腹泻的影响。在GG存在的情况下，Bb12在健康婴儿中的黏附率从31%增加到39%（P = 0.018），在腹泻发作时从26%增加到44%（P = 0.001）。轮状病毒腹泻不会降低粪便黏蛋白的产生，也不会影响体外测试的益生菌的黏附。特定益生菌菌株的组合可能以协同方式增强黏附。这些相互作用的最佳临床应用可能为胃肠道感染的治疗和预防提供新的治疗指导。
