Cafiso D S
Methods Enzymol. 1989;172:331-45. doi: 10.1016/s0076-6879(89)72022-x.
In this article, we have described the use of paramagnetic hydrophobic ions and secondary alkylamines to estimate pH gradients, and transmembrane and interfacial boundary potentials in membrane vesicle systems. Several features of these probes make them powerful tools for the investigation of membrane potentials. The mechanisms by which they function are well understood, thereby facilitating their use in a quantitative fashion. They have a relatively high sensitivity and are not affected by turbidity. While they are ideally suited for use in model membrane systems, they have also been used successfully in biological and reconstituted membrane systems. Finally, the synthetic flexibility of the nitroxide permits a much wider range of probes to be synthesized than has been described here. Future work will likely bring additional developments in the application and information obtainable from this methodology.
在本文中，我们描述了使用顺磁性疏水离子和仲烷基胺来估计膜囊泡系统中的pH梯度、跨膜和界面边界电位。这些探针的几个特性使其成为研究膜电位的有力工具。它们的作用机制已得到充分理解，从而便于以定量方式使用。它们具有相对较高的灵敏度，不受浊度影响。虽然它们非常适合用于模型膜系统，但也已成功应用于生物膜系统和重组膜系统。最后，氮氧化物的合成灵活性使得能够合成比本文所述范围更广的探针。未来的工作可能会在该方法的应用和可获得的信息方面带来更多进展。
