• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过自旋标记疏水离子测量跨磷脂囊泡的电致H⁺/OH⁻移动。

Electrogenic H+/OH- movement across phospholipid vesicles measured by spin-labeled hydrophobic ions.

作者信息

Cafiso D S, Hubbell W L

出版信息

Biophys J. 1983 Oct;44(1):49-57. doi: 10.1016/S0006-3495(83)84276-3.

DOI:10.1016/S0006-3495(83)84276-3
PMID:6313085
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1434809/
Abstract

Transmembrane pH gradients created across phospholipid vesicles give rise to time-dependent potentials as determined from the EPR spectra of phosphonium ion spin labels in the system. From the time-dependent spectra, the transmembrane H+/OH- current is obtained and hence the current-voltage curve for the vesicle membrane is obtained. The current-voltage curve is linear with a membrane resistance of 3 +/- 2 X 10(9) omega cm2 corresponding to a membrane permeability of 5 +/- 2 X 10(-7) cm/s. This unusually high permeability is further increased by small amounts of lipid oxidation, CHCl3 or the general anesthetic halothane.

摘要

跨磷脂囊泡形成的跨膜pH梯度会产生随时间变化的电位，这可通过该系统中鏻离子自旋标记的电子顺磁共振光谱来测定。从随时间变化的光谱中，可以获得跨膜H⁺/OH⁻电流，从而得到囊泡膜的电流-电压曲线。电流-电压曲线呈线性，膜电阻为3±2×10⁹Ω·cm²，对应膜渗透率为5±2×10⁻⁷cm/s。少量的脂质氧化、CHCl₃或全身麻醉药氟烷会进一步提高这种异常高的渗透率。

相似文献

1
Electrogenic H+/OH- movement across phospholipid vesicles measured by spin-labeled hydrophobic ions.通过自旋标记疏水离子测量跨磷脂囊泡的电致H⁺/OH⁻移动。
Biophys J. 1983 Oct;44(1):49-57. doi: 10.1016/S0006-3495(83)84276-3.
2
Transmembrane electrical currents of spin-labeled hydrophobic ions.自旋标记疏水离子的跨膜电流
Biophys J. 1982 Sep;39(3):263-72. doi: 10.1016/S0006-3495(82)84516-5.
3
Probes of membrane electrostatics: synthesis and voltage-dependent partitioning of negative hydrophobic ion spin labels in lipid vesicles.膜静电探针：脂质体中负性疏水离子自旋标记物的合成及电压依赖性分配
Biophys J. 1993 Mar;64(3):642-53. doi: 10.1016/S0006-3495(93)81423-1.
4
Dependency of delta pH-relaxation across vesicular membranes on the buffering power of bulk solutions and lipids.跨囊泡膜的ΔpH弛豫对本体溶液和脂质缓冲能力的依赖性。
Biophys J. 1986 Aug;50(2):265-76. doi: 10.1016/S0006-3495(86)83460-9.
5
Investigation of surface potential asymmetry in phospholipid vesicles by a spin label relaxation method.利用自旋标记弛豫方法研究磷脂囊泡中的表面电位不对称性。
Biophys J. 1986 Feb;49(2):553-62. doi: 10.1016/S0006-3495(86)83665-7.
6
Characterization of H+/OH- currents in phospholipid vesicles.磷脂囊泡中H⁺/OH⁻电流的表征
J Bioenerg Biomembr. 1987 Oct;19(5):443-55. doi: 10.1007/BF00770029.
7
Electron paramagnetic resonance methods for measuring H+/OH- fluxes across phospholipid vesicles.用于测量跨磷脂囊泡的H⁺/OH⁻通量的电子顺磁共振方法。
Methods Enzymol. 1986;127:502-10. doi: 10.1016/0076-6879(86)27040-8.
8
Estimation of transmembrane pH gradients from phase equilibria of spin-labeled amines.通过自旋标记胺的相平衡估算跨膜pH梯度
Biochemistry. 1978 Sep 5;17(18):3871-7. doi: 10.1021/bi00611a030.
9
An electrical and structural characterization of H+/OH- currents in phospholipid vesicles.磷脂囊泡中H⁺/OH⁻电流的电学和结构表征
Biochemistry. 1986 Apr 22;25(8):2270-6. doi: 10.1021/bi00356a063.
10
Estimation of transmembrane potentials from phase equilibria of hydrophobic paramagnetic ions.通过疏水性顺磁离子的相平衡估算跨膜电位。
Biochemistry. 1978 Jan 10;17(1):187-95. doi: 10.1021/bi00594a028.

引用本文的文献

1
Quantifying proton-induced membrane polarization in single biomimetic giant vesicles.定量测量单仿生巨囊泡中的质子诱导的膜极化。
Biophys J. 2022 Jun 21;121(12):2223-2232. doi: 10.1016/j.bpj.2022.05.041. Epub 2022 May 28.
2
Lipid raft components cholesterol and sphingomyelin increase H+/OH- permeability of phosphatidylcholine membranes.脂筏成分胆固醇和鞘磷脂可增加磷脂酰胆碱膜对H⁺/OH⁻的通透性。
Biochem J. 2006 Sep 15;398(3):485-95. doi: 10.1042/BJ20051620.
3
Recognition of ferric catecholates by FepA.FepA对儿茶酚铁的识别。
J Bacteriol. 2004 Jun;186(11):3578-89. doi: 10.1128/JB.186.11.3578-3589.2004.
4
Effects of bilayer surface charge density on molecular adsorption and transport across liposome bilayers.双层表面电荷密度对分子吸附及跨脂质体双层转运的影响。
Biophys J. 2001 Feb;80(2):1004-12. doi: 10.1016/S0006-3495(01)76079-1.
5
Proton transport across transient single-file water pores in a lipid membrane studied by molecular dynamics simulations.通过分子动力学模拟研究质子在脂质膜中瞬态单链水孔中的传输。
Biophys J. 1996 Aug;71(2):632-47. doi: 10.1016/S0006-3495(96)79264-0.
6
A model for self-sustained potential oscillation of lipid bilayer membranes induced by the gel-liquid crystal phase transitions.一种由凝胶 - 液晶相变诱导的脂质双分子层膜自持电位振荡模型。
Biophys J. 1993 May;64(5):1461-75. doi: 10.1016/S0006-3495(93)81513-3.
7
Mechanism of the electric response of lipid bilayers to bitter substances.脂质双分子层对苦味物质电响应的机制。
Biophys J. 1993 Sep;65(3):1219-30. doi: 10.1016/S0006-3495(93)81159-7.
8
Proton/hydroxide conductance through lipid bilayer membranes.质子/氢氧根离子通过脂质双分子层膜的传导
J Membr Biol. 1984;82(1):105-12. doi: 10.1007/BF01870737.
9
The vibrational spectrum of water in liquid alkanes.液态烷烃中水的振动光谱。
Biophys J. 1985 Jul;48(1):117-24. doi: 10.1016/S0006-3495(85)83765-6.
10
Characterization of H+/OH- currents in phospholipid vesicles.磷脂囊泡中H⁺/OH⁻电流的表征
J Bioenerg Biomembr. 1987 Oct;19(5):443-55. doi: 10.1007/BF00770029.

本文引用的文献

1
CHROMATOGRAPHICALLY HOMOGENEOUS LECITHIN FROM EGG PHOSPHOLIPIDS.源自鸡蛋磷脂的色谱纯卵磷脂
J Am Oil Chem Soc. 1965 Jan;42:53-6. doi: 10.1007/BF02558256.
2
Phosphorus assay in column chromatography.柱色谱法中的磷测定
J Biol Chem. 1959 Mar;234(3):466-8.
3
Ion permeability of phospholipid bilayers.磷脂双分子层的离子通透性。
Nature. 1972 Oct 6;239(5371):342-4. doi: 10.1038/239342a0.
4
Phospholipid vesicle formation and transmembrane protein incorporation using octyl glucoside.使用辛基葡糖苷形成磷脂囊泡并整合跨膜蛋白。
Biochemistry. 1981 Feb 17;20(4):833-40. doi: 10.1021/bi00507a028.
5
Transmembrane electrical currents of spin-labeled hydrophobic ions.自旋标记疏水离子的跨膜电流
Biophys J. 1982 Sep;39(3):263-72. doi: 10.1016/S0006-3495(82)84516-5.
6
Proton permeability of liposomes from natural phospholipid mixtures.天然磷脂混合物脂质体的质子渗透性。
Biochim Biophys Acta. 1982 Jan 22;684(2):195-9. doi: 10.1016/0005-2736(82)90005-0.
7
Effect of the lipid phase transition on the kinetics of H+/OH- diffusion across phosphatidic acid bilayers.脂质相变对H⁺/OH⁻跨磷脂酸双层扩散动力学的影响。
Biochim Biophys Acta. 1983 Jan 5;727(1):22-30. doi: 10.1016/0005-2736(83)90364-4.
8
Proton-hydroxide permeability of liposomes.脂质体的质子-氢氧根渗透性。
Proc Natl Acad Sci U S A. 1983 Jan;80(1):165-8. doi: 10.1073/pnas.80.1.165.
9
Proton permeability in biological and model membranes.生物膜和模型膜中的质子渗透性。
Kroc Found Ser. 1981;15:173-87.
10
Proton and hydroxide ion permeability of phospholipid vesicles.磷脂囊泡的质子和氢氧根离子渗透性。
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4324-8. doi: 10.1073/pnas.78.7.4324.