通过自旋标记疏水离子测量跨磷脂囊泡的电致H⁺/OH⁻移动。
Electrogenic H+/OH- movement across phospholipid vesicles measured by spin-labeled hydrophobic ions.
作者信息
Cafiso D S, Hubbell W L
出版信息
Biophys J. 1983 Oct;44(1):49-57. doi: 10.1016/S0006-3495(83)84276-3.
Abstract
Transmembrane pH gradients created across phospholipid vesicles give rise to time-dependent potentials as determined from the EPR spectra of phosphonium ion spin labels in the system. From the time-dependent spectra, the transmembrane H+/OH- current is obtained and hence the current-voltage curve for the vesicle membrane is obtained. The current-voltage curve is linear with a membrane resistance of 3 +/- 2 X 10(9) omega cm2 corresponding to a membrane permeability of 5 +/- 2 X 10(-7) cm/s. This unusually high permeability is further increased by small amounts of lipid oxidation, CHCl3 or the general anesthetic halothane.
摘要
跨磷脂囊泡形成的跨膜pH梯度会产生随时间变化的电位，这可通过该系统中鏻离子自旋标记的电子顺磁共振光谱来测定。从随时间变化的光谱中，可以获得跨膜H⁺/OH⁻电流，从而得到囊泡膜的电流-电压曲线。电流-电压曲线呈线性，膜电阻为3±2×10⁹Ω·cm²，对应膜渗透率为5±2×10⁻⁷cm/s。少量的脂质氧化、CHCl₃或全身麻醉药氟烷会进一步提高这种异常高的渗透率。
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本文引用的文献
1
CHROMATOGRAPHICALLY HOMOGENEOUS LECITHIN FROM EGG PHOSPHOLIPIDS.源自鸡蛋磷脂的色谱纯卵磷脂
J Am Oil Chem Soc. 1965 Jan;42:53-6. doi: 10.1007/BF02558256.
2
Phosphorus assay in column chromatography.柱色谱法中的磷测定
J Biol Chem. 1959 Mar;234(3):466-8.
3
Ion permeability of phospholipid bilayers.磷脂双分子层的离子通透性。
Nature. 1972 Oct 6;239(5371):342-4. doi: 10.1038/239342a0.
4
Phospholipid vesicle formation and transmembrane protein incorporation using octyl glucoside.使用辛基葡糖苷形成磷脂囊泡并整合跨膜蛋白。
Biochemistry. 1981 Feb 17;20(4):833-40. doi: 10.1021/bi00507a028.
5
Transmembrane electrical currents of spin-labeled hydrophobic ions.自旋标记疏水离子的跨膜电流
Biophys J. 1982 Sep;39(3):263-72. doi: 10.1016/S0006-3495(82)84516-5.
6
Proton permeability of liposomes from natural phospholipid mixtures.天然磷脂混合物脂质体的质子渗透性。
Biochim Biophys Acta. 1982 Jan 22;684(2):195-9. doi: 10.1016/0005-2736(82)90005-0.
7
Effect of the lipid phase transition on the kinetics of H+/OH- diffusion across phosphatidic acid bilayers.脂质相变对H⁺/OH⁻跨磷脂酸双层扩散动力学的影响。
Biochim Biophys Acta. 1983 Jan 5;727(1):22-30. doi: 10.1016/0005-2736(83)90364-4.
8
Proton-hydroxide permeability of liposomes.脂质体的质子-氢氧根渗透性。
Proc Natl Acad Sci U S A. 1983 Jan;80(1):165-8. doi: 10.1073/pnas.80.1.165.
9
Proton permeability in biological and model membranes.生物膜和模型膜中的质子渗透性。
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10
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