Cafiso D S, Hubbell W L
Biochemistry. 1978 Sep 5;17(18):3871-7. doi: 10.1021/bi00611a030.
Spin-labeled secondary amines have been used to measure transmembrane proton gradients in sonicated liposomes. The electron paramagnetic resonance spectra of these probes show changes in the ratio of membrane associated to free aqueous probe as a function of transmembrane pH gradient. As the pH gradient is increased, inside acidic, the amount of membrane associated probe increases. The results are accounted for by a simple thermodynamic theory.
自旋标记仲胺已被用于测量超声处理脂质体中的跨膜质子梯度。这些探针的电子顺磁共振光谱显示，膜结合探针与游离水相探针的比例随跨膜pH梯度而变化。随着pH梯度增加（内部呈酸性），膜结合探针的量增加。结果可用一个简单的热力学理论来解释。
