Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, Lagos, Nigeria.
Department of Biochemistry and Microbiology, Nelson Mandela Metropolitan University, Port Elizabeth 6031, South Africa.
J Ethnopharmacol. 2015 Jan 15;159:257-61. doi: 10.1016/j.jep.2014.11.023. Epub 2014 Nov 22.
Enterolobium cyclocarpum (Jacq.) Griseb. is a tropical tree that has folkloric implications against many ailments and diseases including cancer.
To explore the ethnopharmacological claims against cancer, the cytotoxicity of the methanolic extract of the leaves, was investigated using the brine shrimp lethality assay, MTT assay using cervical (HeLa) and breast (MCF7) cancer cell lines, cell cycle analysis and Annexin V-FITC/PI assay.
In the brine shrimp lethality assay, the extract showed cytotoxic activity with LC50 value of 31.63 µg/mL. Significant growth inhibition was observed in both cell lines with IC50 values of 2.07 ± 1.30 µg/mL and 11.84 ± 1.18 µg/mL for HeLa and MCF7, respectively. Cell cycle analysis indicated that HeLa cells were arrested in the G2/M phase while MCF7 cells arrested in the G1/G0 phase. The Annexin V-FITC/PI assay revealed phosphatidylserine translocation in both cell lines and thus apoptosis induction upon treatment with the extract.
The study demonstrated the potential antiproliferative activity of Enterolobium cyclocarpum thereby supporting the traditional claim and provides basis for further mechanistic studies and isolation of active constituents.
环翅山绿豆（Jacq.）Griseb. 是一种热带树木，具有针对多种疾病和病症的民间传说，包括癌症。
为了探索针对癌症的民族药理学说法，使用盐水虾致死性测定法、宫颈（HeLa）和乳腺（MCF7）癌细胞系的 MTT 测定法、细胞周期分析和 Annexin V-FITC/PI 测定法，研究了叶甲醇提取物的细胞毒性。
在盐水虾致死性测定中，提取物表现出细胞毒性，LC50 值为 31.63 µg/mL。在两种细胞系中均观察到显著的生长抑制，HeLa 和 MCF7 的 IC50 值分别为 2.07 ± 1.30 µg/mL 和 11.84 ± 1.18 µg/mL。细胞周期分析表明 HeLa 细胞被阻滞在 G2/M 期，而 MCF7 细胞被阻滞在 G1/G0 期。Annexin V-FITC/PI 测定法显示两种细胞系均发生了磷脂酰丝氨酸易位，因此提取物诱导了细胞凋亡。
该研究表明环翅山绿豆具有潜在的抗增殖活性，从而支持了传统说法，并为进一步的机制研究和活性成分的分离提供了依据。
