Choudhury Bhaswati, Kandimalla Raghuram, Bharali Rupjyoti, Monisha Javadi, Kunnumakara Ajaikumar B, Kalita Kasturi, Kotoky Jibon
Drug Discovery Laboratory, Life Sciences Division , Institute of Advanced Study in Science and Technology Guwahati, India.
Department of Biotechnology, Gauhati University Guwahati, India.
Front Pharmacol. 2016 Jan 29;7:3. doi: 10.3389/fphar.2016.00003. eCollection 2016.
Traditional knowledge (TK) based medicines have gained worldwide attention and presently the scientific community is focussing on proper pharmacological validation and identification of lead compounds for the treatment of various diseases. The North East region of India is the home of valuable traditional herbal remedies. Garcinia morella Desr. (Guttiferae) is one such medicinal plant used by traditional healers for the treatment of inflammatory disorders. The present study was aimed to evaluate the antioxidant and anticancer activity of methanol extracts of the leaf, bark and fruit of G. morella (GM) in different in vitro and in vivo experimental conditions. The results of this study showed that GM methanol extracts possessed in vitro antioxidant and anticancer properties, where the fruit extract (GF) showed maximum activity. The anticancer activity was further confirmed by the results of in vivo administration of GF (200 mg/kg) for ten days to Dalton's lymphoma (DLA) induced mice. GF extract significantly increased the mean survival time (MST) of the animals, decreased the tumor volume and restored the hematological and biochemical parameters. The present study for the first time reported the anticancer property of GF on DLA. Further from the experiments conducted to elucidate the mechanism of action of GF on DLA, it can be concluded that GF exerts its anticancer effect through induction of caspases and DNA fragmentation that ultimately leads to apoptosis. However, further experimentation is required to elucidate the active principle and validate these findings in various in vivo settings.
基于传统知识（TK）的药物已受到全球关注，目前科学界正专注于对用于治疗各种疾病的先导化合物进行适当的药理学验证和鉴定。印度东北地区是宝贵传统草药疗法的发源地。藤黄（藤黄科）就是这样一种药用植物，传统治疗师用它来治疗炎症性疾病。本研究旨在评估藤黄（GM）叶、树皮和果实的甲醇提取物在不同体外和体内实验条件下的抗氧化和抗癌活性。本研究结果表明，GM甲醇提取物具有体外抗氧化和抗癌特性，其中果实提取物（GF）表现出最大活性。通过对道尔顿淋巴瘤（DLA）诱导的小鼠体内给予GF（200mg/kg）十天的结果，进一步证实了其抗癌活性。GF提取物显著延长了动物的平均存活时间（MST），减小了肿瘤体积，并恢复了血液学和生化参数。本研究首次报道了GF对DLA的抗癌特性。通过进一步开展实验以阐明GF对DLA的作用机制，可以得出结论，GF通过诱导半胱天冬酶和DNA片段化发挥其抗癌作用，最终导致细胞凋亡。然而，需要进一步的实验来阐明其活性成分，并在各种体内环境中验证这些发现。
