蔓荆子提取物的细胞毒性活性。

Cytotoxic activity of Vitex trifolia purpurea extracts.

作者信息

El-Sayed Mortada M, El-Hashash Maher M, Mohamed Mona A, Korany Tamer M

机构信息

Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Imbaba P.O.Box 30, Giza, Egypt.

出版信息

J Egypt Soc Parasitol. 2011 Aug;41(2):409-16.

PMID:21980779

Abstract

Defatted 85% crude hot aqueous methanol extract of Vitex trifolia purpurea (AME) successively extracted with, chloroform and ethyl acetate. Cytotoxicity of (AME), chloroform methanol extract (CE), ethyl acetate methanol extract (EE) and the residue obtained from methanol extract after successive extraction (RME) have been evaluated on brine shrimp (Artemia salina) and Hep-G2 cell lines as well. In brine shrimp lethality bioassay the results revealed that the (RME) is the most potent one with LC50 value 173 microg/ml while LC50 values of (AME), (CE) and (EE) was 180, 199 and 286 microg/ml, respectively. As well as the results of cytotoxic assay against Hep-G2 cell lines are in full agreement with previous results, with IC50 values 6, 10.7, 20.8 and 65.8 microg/ml for (RME), (AME), (CE) and (EE), respectively.

摘要

对紫花蔓荆（AME）的85%脱脂粗热甲醇水提取物依次用氯仿和乙酸乙酯进行萃取。对AME、氯仿甲醇提取物（CE）、乙酸乙酯甲醇提取物（EE）以及连续萃取后甲醇提取物得到的残渣（RME），在卤虫（卤虫）和Hep - G2细胞系上进行了细胞毒性评估。在卤虫致死生物测定中，结果显示RME的效力最强，LC50值为173微克/毫升，而AME、CE和EE的LC50值分别为180、199和286微克/毫升。同样，针对Hep - G2细胞系的细胞毒性测定结果与先前结果完全一致，RME、AME、CE和EE的IC50值分别为6、10.7、20.8和65.8微克/毫升。