提高甘油生产丙酸的方法：结合周期性分批发酵和顺序分批发酵以及最佳营养组成。
Improved propionic acid production from glycerol: combining cyclic batch- and sequential batch fermentations with optimal nutrient composition.
机构信息
Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
出版信息
Bioresour Technol. 2015 Jan;176:80-7. doi: 10.1016/j.biortech.2014.11.013. Epub 2014 Nov 11.
Propionic acid was produced from glycerol using Propionibacterium acidipropionici. In this study, the impact of the concentrations of carbon and nitrogen sources, and of different modes of high cell density fermentations on process kinetics and -efficiency was investigated. Three-way ANOVA analysis and batch cultivations at varying C/N ratios at pH 6.5 revealed that propionic acid production rate is significantly influenced by yeast extract concentration. Glycerol to yeast extract ratio (ww(-1)) of 3:1 was required for complete glycerol consumption, while maintaining the volumetric productivity. Using this optimum C/N ratio for propionic acid production in cyclic batch fermentation gave propionate yield up to 93mol% and productivity of 0.53gL(-1)h(-1). Moreover, sequential batch fermentation with cell recycling resulted in production rates exceeding 1gL(-1)h(-1) at initial glycerol up to 120gL(-1), and a maximum of 1.63gL(-1)h(-1) from 90gL(-1) glycerol.
采用丙酸短杆菌从甘油生产丙酸。在这项研究中，考察了不同浓度的碳源和氮源以及不同的高密度发酵方式对过程动力学和效率的影响。三因素方差分析和在 pH 值 6.5 时不同 C/N 比的分批培养表明，丙酸的生产速率受酵母提取物浓度的显著影响。为了完全消耗甘油并保持体积生产率，需要将甘油与酵母提取物的比例（ww(-1)）保持在 3:1。在循环分批发酵中使用该最佳 C/N 比用于丙酸生产，可使丙酸产率高达 93mol%，生产强度达到 0.53gL(-1)h(-1)。此外，采用细胞循环的分批补料发酵，在初始甘油高达 120gL(-1)时，生产速率超过 1gL(-1)h(-1)，从 90gL(-1)甘油可达到最高的 1.63gL(-1)h(-1)。
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