伪狂犬病病毒立即早期基因的DNA核苷酸序列分析
DNA nucleotide sequence analysis of the immediate-early gene of pseudorabies virus.
作者信息
Cheung A K
机构信息
USDA, National Animal Disease Center, Ames, IA 50010.
出版信息
Nucleic Acids Res. 1989 Jun 26;17(12):4637-46. doi: 10.1093/nar/17.12.4637.
The complete DNA sequence coding for the immediate-early protein (IE180) of pseudorabies virus was determined. The coding region of IE180 is 4380 nucleotides for 1460 amino acid residues. G+C content of the non-coding portion of the IE gene is 70.3% while the G+C content of the coding portion is considerably higher at 80.1%. Correspondingly, codons consisting mainly of Gs and Cs are favoured. Clusters of amino acid homologies are observed among IE180 of pseudorabies virus, ICP4 of herpes simplex virus type-1 and IE140 of varicella-zoster virus, and are organized similarly in all three polypeptides. Functions exhibited by IE180 are assigned, tentatively, to structural domains of the molecule by analogy to the HSV-1 ICP4 polypeptide.
确定了伪狂犬病病毒立即早期蛋白（IE180）的完整DNA序列。IE180的编码区为4380个核苷酸，对应1460个氨基酸残基。IE基因非编码部分的G+C含量为70.3%，而编码部分的G+C含量则高得多，为80.1%。相应地，主要由G和C组成的密码子更受青睐。在伪狂犬病病毒的IE180、单纯疱疹病毒1型的ICP4和水痘-带状疱疹病毒的IE140之间观察到氨基酸同源簇，并且在所有三种多肽中组织方式相似。通过与单纯疱疹病毒1型ICP4多肽类比，暂时将IE180表现出的功能分配给该分子的结构域。
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