Molecular Pathology Laboratory, Institute of Molecular Biosciences, Mahidol University, Bangkok, Thailand.
Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Bangkok, Thailand.
Virology. 2014 Dec;471-473:61-71. doi: 10.1016/j.virol.2014.10.004. Epub 2014 Oct 25.
Dengue is the most significant arthropod borne viral disease worldwide, and infection with the dengue virus causes a wide range of symptoms in humans, including bone marrow suppression. While the target cells of the virus remain poorly characterized, cells of the myeloid lineage have been shown to be important mediators of the disease. This study sought to determine whether erythroid precursor cells were susceptible to dengue virus infection, and whether erythroid cells from thalassemia trait carriers showed any protection against infection. Infection with a laboratory adapted high passage DENV-2 resulted in high levels of infection during certain stages of differentiation, and cells derived from thalassemia trait carriers showed significantly reduced susceptibility to dengue virus infection. Infection with low passage isolates resulted in only scattered cells showing evidence of infection, but high bystander apoptosis that was reduced by both a caspase 8 inhibitor and anti-tumor necrosis factor 1 receptor antibodies.
登革热是全球最重要的虫媒病毒病，人类感染登革病毒会引起多种症状，包括骨髓抑制。虽然病毒的靶细胞仍未得到很好的描述，但髓系细胞已被证明是该疾病的重要介质。本研究旨在确定红系前体细胞是否易感染登革病毒，以及携带地中海贫血特征的红细胞是否对感染有任何保护作用。感染实验室适应的高传代 DENV-2 在某些分化阶段会导致高水平的感染，而来自地中海贫血特征携带者的细胞对登革病毒感染的敏感性显著降低。感染低传代分离株只会导致少数细胞出现感染的证据，但高旁观者细胞凋亡可被 caspase 8 抑制剂和抗肿瘤坏死因子 1 受体抗体减少。
