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一种新的水质毒性生物测试——酵母酿酒酵母电导测试法。

A new biological test of water toxicity-yeast Saccharomyces cerevisiae conductometric test.

机构信息

Central Military Hospital - Military Faculty Hospital Prague, U Vojenské nemocnice 1200, 169 02 Praha 6, Czech Republic.

Central Military Hospital - Military Faculty Hospital Prague, U Vojenské nemocnice 1200, 169 02 Praha 6, Czech Republic.

出版信息

Environ Toxicol Pharmacol. 2014 Nov;38(3):977-81. doi: 10.1016/j.etap.2014.10.009. Epub 2014 Oct 18.

Abstract

This new biological test of water toxicity is based on monitoring of specific conductivity changes of yeast Saccharomyces cerevisiae suspension as a result of yeast fermentation activity inhibition in toxic conditions. The test was verified on ten substances with various mechanisms of toxic effect and the results were compared with two standard toxicity tests based on Daphnia magna mobility inhibition (EN ISO 6341) and Vibrio fischeri bioluminescence inhibition (EN ISO 11348-2) and with the results of the S. cerevisiae lethal test (Rumlova and Dolezalova, 2012). The new biological test - S. cerevisiae conductometric test - is an express method developed primarily for field conditions. It is applicable in case of need of immediate information about water toxicity. Fast completion is an advantage of this test (time necessary for test completion is about 60min), the test is simple and the test organism - dried instant yeast - belongs among its biggest advantages because of its long-term storage life and broad availability.

摘要

这种新的水质毒性生物测试是基于监测酵母酿酒酵母悬浮液的特定电导率变化,因为在有毒条件下酵母发酵活动受到抑制。该测试已在十种具有不同毒性作用机制的物质上进行了验证,并将结果与两种基于大型溞移动抑制的标准毒性测试(EN ISO 6341)和发光细菌发光抑制(EN ISO 11348-2)进行了比较,并与酿酒酵母致死测试(Rumlova 和 Dolezalova,2012)的结果进行了比较。新的生物测试——酿酒酵母电导测试——是一种主要为现场条件开发的快速方法。在需要立即了解水质毒性的情况下,它是适用的。快速完成是该测试的优势(完成测试所需的时间约为 60 分钟),该测试简单,测试生物——即发干酵母——因其长期储存寿命和广泛可用性而成为其最大优势之一。

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