University of Pannonia, Institute of Environmental Sciences, Egyetem str. 10, 8200 Veszprém, Hungary.
University of Pannonia, Institute of Environmental Sciences, Egyetem str. 10, 8200 Veszprém, Hungary.
Ecotoxicol Environ Saf. 2018 Apr 30;151:170-177. doi: 10.1016/j.ecoenv.2018.01.015. Epub 2018 Feb 4.
Biodiesel is considered an important renewable energy source but still there is some controversy about its environmental toxicity, especially to aquatic life. In our study, the toxicity of water soluble fraction of biodiesel was evaluated in relatively low concentrations using a battery of bioassays: Vibrio fischeri bioluminescence inhibition, Sinapis alba root growth inhibition, Daphnia magna immobilization, boar semen live/dead ratio and DNA fragmentation and Unio pictorum micronucleus test. While the S. alba test indicated nutritive (stimulating) effect of the sample, the biodiesel exerted toxic effect in the aquatic tests. D. magna was the most sensitive with EC value of 0.0226%. For genotoxicity assessment, the mussel micronucleus test (MNT) was applied, detecting considerable genotoxic potential of the biodiesel sample: it elucidated micronuclei formation already at low concentration of 3.3%. Although this test has never been employed in biodiesel eco/genotoxicity assessments, it seems a promising tool, based on its appropriate sensitivity, and representativity.
生物柴油被认为是一种重要的可再生能源，但它的环境毒性，特别是对水生生物的毒性仍存在一些争议。在我们的研究中，使用一系列生物测定法评估了相对低浓度下生物柴油水溶性馏分的毒性：发光菌的生物发光抑制、油菜根生长抑制、大型蚤的固定、公猪精液的死活比和 DNA 片段化以及圆田螺的微核试验。虽然油菜根试验表明样品具有营养（刺激）作用，但生物柴油在水生测试中表现出毒性作用。大型蚤对 EC 值为 0.0226%最敏感。对于遗传毒性评估，应用了贻贝微核试验（MNT），检测到生物柴油样品具有相当大的遗传毒性潜力：它已经在低浓度 3.3%下阐明了微核的形成。尽管该试验从未用于生物柴油的生态/遗传毒性评估，但它似乎是一种很有前途的工具，基于其适当的敏感性和代表性。
