School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea.
J Hazard Mater. 2015 Mar 2;284:261-8. doi: 10.1016/j.jhazmat.2014.11.021. Epub 2014 Nov 20.
Exiguobacterium sp. PMA utilized 4-chloroindole as its sole source of carbon and energy. The effect of initial concentrations of substrate on the 4-chloroindole degradation was studied and observed that strain PMA was capable of degrading 4-chloroindole up to concentration of 0.5mM. The degradation pathway of 4-chloroindole was studied for Exiguobacterium sp. PMA based on metabolites identified by gas chromatography-mass spectrometry. 4-Chloroindole was initially dehalogenated to indole that was further degraded via isatin, anthranilic acid, and salicylic acid. The potential of strain PMA to degrade 4-chloroindole in soil was monitored using soil microcosms, and it was observed that the cells of strain PMA efficiently degraded 4-chloroindole in the soil. The results of microcosm studies show that strain PMA may be used for bioremediation of 4-chloroindole-contaminated sites. This is the first report of the bacterial degradation of 4-chloroindole.
极端芽孢杆菌 PMA 将 4-氯吲哚作为其唯一的碳源和能源加以利用。本研究考察了初始底物浓度对 4-氯吲哚降解的影响，结果表明，PMA 菌株能够降解高达 0.5mM 的 4-氯吲哚。基于气相色谱-质谱鉴定的代谢物，研究了极端芽孢杆菌 PMA 对 4-氯吲哚的降解途径。4-氯吲哚首先脱卤化为吲哚，然后通过色氨酸、邻氨基苯甲酸和水杨酸进一步降解。采用土壤微宇宙法监测了 PMA 菌株在土壤中降解 4-氯吲哚的能力，结果表明 PMA 菌株能够有效地在土壤中降解 4-氯吲哚。微宇宙研究结果表明，PMA 菌株可用于生物修复 4-氯吲哚污染场所。这是细菌降解 4-氯吲哚的首例报道。
