Chen Qingguo, Li Jingjing, Liu Mei, Sun Huiling, Bao Mutai
College of Marine Science & Technology, Zhejiang Ocean University, Zhoushan, P. R. China.
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao, P. R. China.
PLoS One. 2017 Mar 27;12(3):e0174445. doi: 10.1371/journal.pone.0174445. eCollection 2017.
Five strains of bacteria, namely, Exiguobacterium sp. ASW-1, Pseudomonas aeruginosa strain ASW-2, Alcaligenes sp. ASW-3, Alcaligenes sp. ASS-1, and Bacillus sp. ASS-2, were isolated from the Zhejiang coast in China. The mixed flora of the five strains performed well with degrading 75.1% crude oil (1%, w/v) in 7 days. The calcium alginate-activated carbon embedding carrier was used to immobilize bacterial consortium. Immobilized cells performed better than free ones in variations of environmental factors containing incubated temperature, initial pH, salinity of the medium and crude oil concentration. The degradation process of crude oil by immobilized bacteria was accelerated compared with that of the free ones. Bacterial consortium showed better performance on biodegradation of normal alkanes than that of PAHs. Improvement of immobilization on the biodegradation efficiency of normal alkanes (31.9%) was apparently high than that of PAHs (1.9%).
从中国浙江海岸分离出五株细菌，分别为嗜麦芽寡养单胞菌ASW-1、铜绿假单胞菌ASW-2、产碱杆菌ASW-3、产碱杆菌ASS-1和芽孢杆菌ASS-2。这五株菌的混合菌群在7天内对1%（w/v）的原油降解率达75.1%，表现良好。采用海藻酸钙活性炭包埋载体固定化细菌菌群。在包括培养温度、初始pH值、培养基盐度和原油浓度在内的环境因素变化中，固定化细胞比游离细胞表现更好。与游离细菌相比，固定化细菌对原油的降解过程加快。细菌菌群对正构烷烃的生物降解性能优于多环芳烃。固定化对正构烷烃生物降解效率的提高（31.9%）明显高于多环芳烃（1.9%）。
