Tóth F, Nissi M J, Wang L, Ellermann J M, Carlson C S
Veterinary Population Medicine Department, University of Minnesota, St. Paul, MN, USA.
Center for Magnetic Resonance Research, Department of Radiology, University of Minnesota, 2021 Sixth Street SE, Minneapolis, MN 55455, United States; Department of Orthopaedic Surgery, University of Minnesota, Minneapolis, MN, USA.
Osteoarthritis Cartilage. 2015 Feb;23(2):300-7. doi: 10.1016/j.joca.2014.11.009. Epub 2014 Nov 15.
Identify and interrupt the vascular supply to portions of the distal femoral articular-epiphyseal cartilage complex (AECC) in goat kids to induce cartilage necrosis, characteristic of early lesions of osteochondrosis (OC); then utilize magnetic resonance imaging (MRI) to identify necrotic areas of cartilage.
Distal femora were perfused and cleared in goat kids of various ages to visualize the vascular supply to the distal femoral AECC. Vessels located on the axial aspect of the medial femoral condyle (MFC) and on the abaxial side of the lateral trochlear ridge were transected in eight 4- to 5-day-old goats to induce cartilage necrosis. Goats were euthanized 1, 2, 3, 4, 5, 6, 9, and 10 weeks post operatively and operated stifles were harvested. Adiabatic T1ρ relaxation time maps of the harvested distal femora were generated using a 9.4 T MR scanner, after which samples were evaluated histologically.
Interruption of the vascular supply to the MFC caused lesions of cartilage necrosis in 6/8 goat kids that were demonstrated histologically. Adiabatic T1ρ relaxation time mapping identified these areas of cartilage necrosis in 5/6 cases. No significant findings were detected after transection of perichondrial vessels supplying the lateral trochlear ridge.
Cartilage necrosis, characteristic of early OC, can be induced by interrupting the vascular supply to the distal femoral AECC in goat kids. The ability of high field MRI to identify these areas of cartilage necrosis in the AECC using the adiabatic T1ρ sequence suggests that this technique may be useful in the future for the early diagnosis of OC.
识别并阻断山羊幼崽股骨远端关节-骨骺软骨复合体（AECC）部分区域的血管供应，以诱导软骨坏死，这是骨软骨病（OC）早期病变的特征；然后利用磁共振成像（MRI）识别软骨坏死区域。
对不同年龄的山羊幼崽的股骨远端进行灌注和清理，以观察股骨远端AECC的血管供应。在8只4至5日龄的山羊中，切断位于内侧股骨髁（MFC）轴侧和外侧滑车嵴背侧的血管，以诱导软骨坏死。术后1、2、3、4、5、6、9和10周对山羊实施安乐死，并采集手术侧的 stifle（未明确的解剖部位，可能是膝关节相关结构）。使用9.4 T MR扫描仪生成采集的股骨远端的绝热T1ρ弛豫时间图，之后对样本进行组织学评估。
切断MFC的血管供应导致6/8只山羊幼崽出现软骨坏死病变，组织学检查证实了这一点。绝热T1ρ弛豫时间成像在5/6例中识别出了这些软骨坏死区域。切断供应外侧滑车嵴的软骨膜血管后未发现明显异常。
通过阻断山羊幼崽股骨远端AECC的血管供应可诱导出OC早期病变特征性的软骨坏死。高场MRI利用绝热T1ρ序列识别AECC中这些软骨坏死区域的能力表明，该技术未来可能有助于OC的早期诊断。
