Liu Yuan, Nishimoto Mamoru, Kitaoka Motomitsu
National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
Carbohydr Res. 2015 Jan 12;401:1-4. doi: 10.1016/j.carres.2014.10.014. Epub 2014 Oct 25.
Three sugar 1-phosphates that are donor substrates for phosphorylases were produced at the gram scale from phosphoenolpyruvic acid and the corresponding sugars by the combined action of pyruvate kinase and the corresponding anomeric kinases in good yields. These sugar 1-phosphates were purified through two electrodialysis steps. α-D-Galactose 1-phosphate was finally isolated as crystals of dipotassium salts. α-D-Mannose 1-phosphate and 2-acetamido-2-deoxy-α-D-glucose 1-phosphate were isolated as crystals of bis(cyclohexylammonium) salts.
通过丙酮酸激酶和相应异头物激酶的联合作用，以磷酸烯醇丙酮酸和相应的糖为原料，以良好的产率在克级规模上制备了三种作为磷酸化酶供体底物的糖1-磷酸酯。这些糖1-磷酸酯通过两步电渗析步骤进行纯化。α-D-半乳糖1-磷酸最终以二钾盐晶体形式分离出来。α-D-甘露糖1-磷酸和2-乙酰氨基-2-脱氧-α-D-葡萄糖1-磷酸以双（环己基铵）盐晶体形式分离出来。
