Yao Wenlong, Yan Jun, Chen Xi, Wang Fengshan, Cao Hongzhi
National Glycoengineering Research Center, School of Pharmaceutical Science, Shandong University, Jinan 250012, China.
Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA.
Carbohydr Res. 2015 Jan 12;401:5-10. doi: 10.1016/j.carres.2014.10.017. Epub 2014 Oct 29.
A concise and practical chemoenzymatic synthesis of lacto-N-tetraose, a major component and one of the most common core structures of human milk oligosaccharides (HMOs) was reported. This convergent synthesis relies on the glycosylation of a readily available lactoside acceptor with a lacto-N-biose donor generated from a highly efficient one-pot two-enzyme synthesis.
报道了一种简洁实用的化学酶法合成乳糖 - N - 四糖的方法，乳糖 - N - 四糖是母乳低聚糖（HMOs）的主要成分和最常见的核心结构之一。这种汇聚式合成依赖于一种易于获得的乳糖苷受体与通过高效一锅双酶合成产生的乳糖 - N - 二糖供体之间的糖基化反应。
