Soranzo C, Ingrosso A, Pratesi G, Lombardi L, Pilotti S, Zunino F
Division of Experimental Oncology B, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milano, Italy.
Anticancer Res. 1989 Mar-Apr;9(2):361-6.
Malignant transformation of mouse host cells by a human small cell lung cancer (SCLC) was demonstrated by short-term in vitro cultivation of the tumor cells from a xenograft at two different transplant generations. Isoenzyme (LDH) and chromosome analysis showed that out of the 3 cell lines established from this tumor, 1 retained a human karyotype similar to that of the xenograft and 2 were murine transformed cell lines. These murine cell lines produced fibro-sarcoma-like tumors when injected into nude mice. Because of the early in vitro emergence of murine transformed cell populations, it is likely that the transformation process had occurred in vivo. Since in our experience the induction of transformation of host murine cells, also observed directly in vivo, is more frequent with SCLC than other histotypes (lung and colorectal adenocarcinoma), it is suggested that the known production of growth factor by these tumors may contribute to this transformation.
通过对来自异种移植瘤在两个不同移植代次的肿瘤细胞进行短期体外培养，证明了人小细胞肺癌（SCLC）可使小鼠宿主细胞发生恶性转化。同工酶（LDH）和染色体分析表明，从该肿瘤建立的3个细胞系中，1个保留了与异种移植瘤相似的人类核型，2个是小鼠转化细胞系。将这些小鼠细胞系注射到裸鼠体内时会产生纤维肉瘤样肿瘤。由于在体外早期出现了小鼠转化细胞群体，因此转化过程很可能在体内已经发生。根据我们的经验，与其他组织学类型（肺和结肠直肠腺癌）相比，SCLC在体内也能直接观察到更频繁地诱导宿主小鼠细胞发生转化，提示这些肿瘤已知产生的生长因子可能促成了这种转化。
