Zhang S Y, Dai Z Q, Shu Y H, Zhang J L, Xu B, Lin Z Q, Wu S F, Liu Y F, Xu L Z
Shanghai Institute of Materia Medica, Chinese Academy of Sciences.
Zhonghua Zhong Liu Za Zhi. 1987 Sep;9(5):333-5, 20.
A human lung oat cell carcinoma from a surgical specimen was successfully transplanted into nude mice and has been maintained for over four years through 14 passages. This transplanted tumor had a long latent period of more than one month, slowly growing in the initial inoculation and the following passages. The interval between two passages was about four months. 5 months after the inoculation, the transplanted tumor in nude mice grew to 2 cm in diameter. It was demonstrated that the transplanted tumor possessed the human isoenzyme pattern and human chromosome karyotype. The original human tumor and its transplanted counterparts in the nude mice were strikingly similar by histopathology. Besides, specific neuro-secretory granules in cytoplasm of the transplanted tumor were observed under transmission electron microscope.
从手术标本中获取的一株人肺燕麦细胞癌成功移植到裸鼠体内，并通过14次传代维持了四年多。这种移植瘤有一个多月的较长潜伏期，在初次接种及随后的传代过程中生长缓慢。两次传代之间的间隔约为四个月。接种后5个月，裸鼠体内的移植瘤直径长到了2厘米。结果表明，移植瘤具有人同工酶模式和人染色体核型。原代人肿瘤及其在裸鼠体内的移植对应物在组织病理学上极为相似。此外，在透射电子显微镜下观察到移植瘤细胞质中有特异性神经分泌颗粒。
