Yamano T, Nonaka Y, Okamoto M, Matsubara T, Miura R
Department of Biochemistry, Osaka University Medical School, Japan.
Biochem Biophys Res Commun. 1989 Jul 14;162(1):168-74. doi: 10.1016/0006-291x(89)91977-3.
The rate of electron transport in the cytochrome P-450 system in adrenocortical mitochondria was studied with purified adrenodoxin reductase, adrenodoxin and cytochrome c. Oxaloacetate enhanced the rate at concentrations of less than 1 mM; malate, succinate and fumarate enhanced the rate to a lesser extent; and pyruvate and alpha-ketoglutarate had no appreciable effect. The rate enhancement was observed when the reagents were preincubated with adrenodoxin, but not with adrenodoxin reductase. Rate enhancement was also evident when the rate limiting step was at adrenodoxin in the electron transport system.
